Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 1234616..1235295 Replicon chromosome
Accession NZ_CP124455
Organism Escherichia coli strain AVS0193

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag QJP76_RS05900 Protein ID WP_000057523.1
Coordinates 1234616..1234918 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag QJP76_RS05905 Protein ID WP_000806442.1
Coordinates 1234954..1235295 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QJP76_RS05875 (1229992) 1229992..1231644 + 1653 WP_000771748.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
QJP76_RS05880 (1231682) 1231682..1232185 - 504 WP_000667000.1 hypothetical protein -
QJP76_RS05885 (1232182) 1232182..1232838 - 657 WP_015674862.1 hypothetical protein -
QJP76_RS05890 (1233006) 1233006..1233485 - 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
QJP76_RS05895 (1233689) 1233689..1234483 - 795 WP_000365147.1 TraB/GumN family protein -
QJP76_RS05900 (1234616) 1234616..1234918 + 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
QJP76_RS05905 (1234954) 1234954..1235295 + 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
QJP76_RS05910 (1235353) 1235353..1237857 - 2505 WP_000083947.1 copper-exporting P-type ATPase CopA -
QJP76_RS05915 (1238119) 1238119..1239051 + 933 WP_000883041.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T280318 WP_000057523.1 NZ_CP124455:1234616-1234918 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT280318 WP_000806442.1 NZ_CP124455:1234954-1235295 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References