Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) hok-sok/-
Location 81886..82125 Replicon plasmid pAVS0790-A
Accession NZ_CP124437
Organism Escherichia coli strain AVS0790

Toxin (Protein)


Gene name srnB Uniprot ID A0A762TWR7
Locus tag QJP66_RS25920 Protein ID WP_023144756.1
Coordinates 81991..82125 (+) Length 45 a.a.

Antitoxin (RNA)


Gene name sok
Locus tag -
Coordinates 81886..81946 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QJP66_RS25890 (77677) 77677..78237 + 561 WP_000139341.1 fertility inhibition protein FinO -
QJP66_RS25895 (78368) 78368..78580 + 213 WP_013023861.1 hypothetical protein -
QJP66_RS25900 (79139) 79139..79564 + 426 WP_000422741.1 transposase -
QJP66_RS25905 (79561) 79561..79911 + 351 WP_000624722.1 IS66 family insertion sequence element accessory protein TnpB -
QJP66_RS25910 (79942) 79942..81555 + 1614 WP_000080195.1 IS66-like element ISEc23 family transposase -
QJP66_RS25915 (81633) 81633..81919 + 287 Protein_99 DUF2726 domain-containing protein -
- (81886) 81886..81946 - 61 NuclAT_2 - Antitoxin
- (81886) 81886..81946 - 61 NuclAT_2 - Antitoxin
- (81886) 81886..81946 - 61 NuclAT_2 - Antitoxin
- (81886) 81886..81946 - 61 NuclAT_2 - Antitoxin
QJP66_RS25920 (81991) 81991..82125 + 135 WP_023144756.1 Hok/Gef family protein Toxin
QJP66_RS25925 (82422) 82422..82676 + 255 WP_000083850.1 replication regulatory protein RepA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-27 / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 - 1..82979 82979


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Hok/gef cell toxic protein

(4-44)


Sequences

Toxin        


Download         Length: 45 a.a.        Molecular weight: 4896.90 Da        Isoelectric Point: 7.7209

>T280280 WP_023144756.1 NZ_CP124437:81991-82125 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Download         Length: 135 bp

>T280280 NZ_CP124437:81991-82125 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTTGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACAGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTGA

Antitoxin


Download         Length: 61 bp

>AT280280 NZ_CP124437:c81946-81886 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A762TWR7

Antitoxin

Download structure file

References