Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 4592331..4593010 Replicon chromosome
Accession NZ_CP124420
Organism Escherichia coli strain AVS0155

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag QJP63_RS22395 Protein ID WP_000057523.1
Coordinates 4592331..4592633 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag QJP63_RS22400 Protein ID WP_000806442.1
Coordinates 4592669..4593010 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QJP63_RS22370 (4587707) 4587707..4589359 + 1653 WP_000771748.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
QJP63_RS22375 (4589397) 4589397..4589900 - 504 WP_000667000.1 hypothetical protein -
QJP63_RS22380 (4589897) 4589897..4590697 - 801 WP_000439798.1 hypothetical protein -
QJP63_RS22385 (4590721) 4590721..4591200 - 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
QJP63_RS22390 (4591404) 4591404..4592198 - 795 WP_000365147.1 TraB/GumN family protein -
QJP63_RS22395 (4592331) 4592331..4592633 + 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
QJP63_RS22400 (4592669) 4592669..4593010 + 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
QJP63_RS22405 (4593068) 4593068..4595572 - 2505 WP_000083947.1 copper-exporting P-type ATPase CopA -
QJP63_RS22410 (4595834) 4595834..4596766 + 933 WP_000883041.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T280171 WP_000057523.1 NZ_CP124420:4592331-4592633 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT280171 WP_000806442.1 NZ_CP124420:4592669-4593010 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References