Detailed information of TA system TA028016

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5343686..5344281
• Replicon: chromosome
• Accession: NC_018080
• Organism: Pseudomonas aeruginosa DK2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: PADK2_RS30620
• Protein ID: WP_003117425.1
• Coordinates: 5344003..5344281 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PADK2_RS24790
• Protein ID: WP_003113527.1
• Coordinates: 5343686..5343991 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PADK2_RS24755	5338826..5339674	+	849	WP_003117426.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
PADK2_RS24765	5339841..5340782	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
PADK2_RS24770	5340899..5341513	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
PADK2_RS24775	5341555..5342139	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
PADK2_RS24780	5342180..5343280	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
PADK2_RS24790	5343686..5343991	-	306	WP_003113527.1	HigA family addiction module antidote protein	Antitoxin
PADK2_RS30620	5344003..5344281	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PADK2_RS24795	5344610..5346838	+	2229	WP_003113525.1	TonB-dependent receptor	-
PADK2_RS24800	5346908..5347555	-	648	WP_003095021.1	carbonate dehydratase	-
PADK2_RS24805	5347617..5348855	-	1239	WP_003117424.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T28016 WP_003117425.1 NC_018080:c5344281-5344003 [Pseudomonas aeruginosa DK2]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T28016 NC_018080:c5344281-5344003 [Pseudomonas aeruginosa DK2]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTAGGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT28016 WP_003113527.1 NC_018080:c5343991-5343686 [Pseudomonas aeruginosa DK2]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT28016 NC_018080:c5343991-5343686 [Pseudomonas aeruginosa DK2]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: A0A241XLJ5