Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 4078551..4079230 Replicon chromosome
Accession NZ_CP124415
Organism Escherichia coli strain AVS0223

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag QJP60_RS19565 Protein ID WP_000057523.1
Coordinates 4078551..4078853 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag QJP60_RS19570 Protein ID WP_000806442.1
Coordinates 4078889..4079230 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QJP60_RS19540 (4073927) 4073927..4075579 + 1653 WP_000771748.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
QJP60_RS19545 (4075617) 4075617..4076120 - 504 WP_000667000.1 hypothetical protein -
QJP60_RS19550 (4076117) 4076117..4076917 - 801 WP_000439798.1 hypothetical protein -
QJP60_RS19555 (4076941) 4076941..4077420 - 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
QJP60_RS19560 (4077624) 4077624..4078418 - 795 WP_000365147.1 TraB/GumN family protein -
QJP60_RS19565 (4078551) 4078551..4078853 + 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
QJP60_RS19570 (4078889) 4078889..4079230 + 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
QJP60_RS19575 (4079288) 4079288..4081792 - 2505 WP_000083947.1 copper-exporting P-type ATPase CopA -
QJP60_RS19580 (4082054) 4082054..4082986 + 933 WP_000883041.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T280132 WP_000057523.1 NZ_CP124415:4078551-4078853 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT280132 WP_000806442.1 NZ_CP124415:4078889-4079230 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References