Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 4103942..4104621 Replicon chromosome
Accession NZ_CP124410
Organism Escherichia coli strain AVS0543

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag QJP61_RS19725 Protein ID WP_000057523.1
Coordinates 4103942..4104244 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag QJP61_RS19730 Protein ID WP_000806442.1
Coordinates 4104280..4104621 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QJP61_RS19700 (4099318) 4099318..4100970 + 1653 WP_000771748.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
QJP61_RS19705 (4101008) 4101008..4101511 - 504 WP_000667000.1 hypothetical protein -
QJP61_RS19710 (4101508) 4101508..4102308 - 801 WP_000439798.1 hypothetical protein -
QJP61_RS19715 (4102332) 4102332..4102811 - 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
QJP61_RS19720 (4103015) 4103015..4103809 - 795 WP_000365147.1 TraB/GumN family protein -
QJP61_RS19725 (4103942) 4103942..4104244 + 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
QJP61_RS19730 (4104280) 4104280..4104621 + 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
QJP61_RS19735 (4104679) 4104679..4107183 - 2505 WP_000083947.1 copper-exporting P-type ATPase CopA -
QJP61_RS19740 (4107445) 4107445..4108377 + 933 WP_000883041.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T280098 WP_000057523.1 NZ_CP124410:4103942-4104244 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT280098 WP_000806442.1 NZ_CP124410:4104280-4104621 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References