Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3782795..3783452 Replicon chromosome
Accession NC_018079
Organism Enterobacter cloacae subsp. dissolvens SDM

Toxin (Protein)


Gene name higB Uniprot ID A0A421IAD7
Locus tag A3UG_RS17690 Protein ID WP_014832979.1
Coordinates 3782795..3783094 (+) Length 100 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A427KGI2
Locus tag A3UG_RS17695 Protein ID WP_014832980.1
Coordinates 3783105..3783452 (+) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A3UG_RS17675 3778401..3780593 + 2193 WP_014832976.1 type I secretion system permease/ATPase -
A3UG_RS17680 3780604..3781773 + 1170 WP_126522669.1 HlyD family efflux transporter periplasmic adaptor subunit -
A3UG_RS24535 3781965..3782079 + 115 Protein_3471 ash family protein -
A3UG_RS17685 3782101..3782538 + 438 WP_014832978.1 hypothetical protein -
A3UG_RS17690 3782795..3783094 + 300 WP_014832979.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
A3UG_RS17695 3783105..3783452 + 348 WP_014832980.1 HigA family addiction module antidote protein Antitoxin
A3UG_RS17700 3783498..3783923 + 426 Protein_3475 nucleoside triphosphatase NudI -
A3UG_RS17705 3783895..3784626 - 732 WP_014832983.1 helix-turn-helix transcriptional regulator -
A3UG_RS17710 3784725..3785144 + 420 WP_014832984.1 nuclear transport factor 2 family protein -
A3UG_RS17715 3785233..3786414 - 1182 WP_014832985.1 PLP-dependent aminotransferase family protein -
A3UG_RS17720 3786434..3787321 - 888 WP_014832986.1 LysR family transcriptional regulator -
A3UG_RS17725 3787419..3788021 + 603 WP_014832987.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(20-71)


Sequences

Toxin        


Download         Length: 100 a.a.        Molecular weight: 11646.28 Da        Isoelectric Point: 10.0182

>T28009 WP_014832979.1 NC_018079:3782795-3783094 [Enterobacter cloacae subsp. dissolvens SDM]
MINHFRDQWLEDFFLYGRSSNVIPSNLETVLARKLDLINAAISHRDLRSPPGNMFEALNPPLKGYSSIRVNRQYRLVFRW
AEGKADDLYLSPHKYTQHK

Download         Length: 300 bp

>T28009 NC_018079:3782795-3783094 [Enterobacter cloacae subsp. dissolvens SDM]
ATGATTAATCATTTTCGGGATCAATGGCTTGAGGATTTTTTTCTTTACGGGAGATCGAGCAATGTTATTCCTTCAAATCT
GGAAACCGTTCTTGCAAGAAAGCTCGACCTCATCAATGCAGCCATCTCGCATCGGGATTTGCGTTCACCACCGGGCAATA
TGTTTGAAGCATTGAATCCGCCGCTGAAGGGATATTCCTCAATCCGGGTAAACAGACAATACAGGCTTGTATTTCGCTGG
GCAGAGGGAAAAGCAGACGATCTCTATCTCTCTCCACACAAGTACACGCAACACAAGTGA

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13066.11 Da        Isoelectric Point: 8.5158

>AT28009 WP_014832980.1 NC_018079:3783105-3783452 [Enterobacter cloacae subsp. dissolvens SDM]
MILQQALRKPTTPGDVLQYEYLEPLNLKISDLAEMLNVHRNTISALVNNNRKLTADMAIKLAKAFDTTIEFWLKLQLNVD
IWEAQSNSRTQEELSRIKTVAEVMAKRKGGQPDVA

Download         Length: 348 bp

>AT28009 NC_018079:3783105-3783452 [Enterobacter cloacae subsp. dissolvens SDM]
ATGATACTTCAACAGGCACTCCGCAAACCCACCACGCCGGGCGACGTGTTGCAATATGAGTATCTTGAACCGCTCAATCT
GAAAATCAGCGATCTGGCGGAGATGCTAAATGTACACCGCAATACCATCAGCGCGCTGGTAAATAACAATCGCAAGCTTA
CCGCCGATATGGCGATAAAATTGGCAAAAGCCTTTGATACCACTATTGAATTTTGGTTGAAATTACAACTGAACGTCGAT
ATCTGGGAAGCACAATCAAACTCCAGAACGCAAGAGGAGCTGAGCCGTATAAAAACCGTTGCGGAAGTCATGGCGAAGCG
TAAAGGTGGTCAACCGGATGTTGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A421IAD7

Antitoxin

Source ID Structure
AlphaFold DB A0A427KGI2

References