Detailed information of TA system TA028008

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3185627..3186287
• Replicon: chromosome
• Accession: NC_018079
• Organism: Enterobacter cloacae subsp. dissolvens SDM

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A3R9FZF4
• Locus tag: A3UG_RS15085
• Protein ID: WP_014832569.1
• Coordinates: 3185913..3186287 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A3UG_RS15080
• Protein ID: WP_014832568.1
• Coordinates: 3185627..3185929 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A3UG_RS15060	3181485..3183674	+	2190	WP_014832565.1	TonB-dependent siderophore receptor	-
A3UG_RS15070	3184068..3184457	-	390	WP_014832566.1	RidA family protein	-
A3UG_RS15075	3184599..3185531	+	933	WP_014832567.1	LysR family transcriptional regulator	-
A3UG_RS15080	3185627..3185929	-	303	WP_014832568.1	XRE family transcriptional regulator	Antitoxin
A3UG_RS15085	3185913..3186287	-	375	WP_014832569.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A3UG_RS15090	3186473..3186679	-	207	Protein_2964	transcriptional regulator	-
A3UG_RS23795	3186701..3186928	+	228	Protein_2965	glutathione S-transferase	-
A3UG_RS15095	3186978..3187928	-	951	WP_014832572.1	HTH-type transcriptional regulator Cbl	-
A3UG_RS15100	3188025..3188942	-	918	WP_014832573.1	nitrogen assimilation transcriptional regulator NAC	-
A3UG_RS15110	3189433..3190329	-	897	WP_043951667.1	L,D-transpeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14358.33 Da
• Isoelectric Point: 9.7405

>T28008 WP_014832569.1 NC_018079:c3186287-3185913 [Enterobacter cloacae subsp. dissolvens SDM]
VWTINTTDRFDCWFRSLDDTDRASVLAALLVLREKGPALSRPYADTLRGSRHSNMKELRIQSKGDPLRAFFAFDPNRTGI
VLCAGNKVGNERRFYDEMLLVADREFTHWLNTLKERTLRDGQKP

Nucleotide

• Length: 375 bp

>T28008 NC_018079:c3186287-3185913 [Enterobacter cloacae subsp. dissolvens SDM]
GTGTGGACAATTAACACGACGGACAGGTTCGATTGCTGGTTTCGTTCACTTGACGATACTGATCGGGCCAGCGTGCTGGC
AGCACTGCTTGTTTTGCGAGAAAAGGGGCCTGCTTTGTCGAGGCCCTATGCCGATACGCTTAGAGGCTCTCGTCACAGCA
ACATGAAAGAGTTGCGCATTCAGAGTAAGGGCGATCCGTTGCGGGCATTTTTCGCGTTTGACCCAAACCGTACCGGCATT
GTGCTCTGTGCCGGCAATAAGGTCGGCAACGAAAGACGCTTTTACGATGAGATGCTTCTGGTGGCCGACCGGGAATTCAC
TCACTGGTTGAATACATTAAAAGAGAGGACGTTACGCGATGGGCAGAAACCTTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 10972.69 Da
• Isoelectric Point: 5.1473

>AT28008 WP_014832568.1 NC_018079:c3185929-3185627 [Enterobacter cloacae subsp. dissolvens SDM]
MGRNLEQLIADEKSDVVASAQAMAADILLNIHLAELREKVQKTQVEMAQALGIRQPTVAVMEKPGRDLKLSSLKRYVEAA
GGKLTLDVELPDGSHYEFVL

Nucleotide

• Length: 303 bp

>AT28008 NC_018079:c3185929-3185627 [Enterobacter cloacae subsp. dissolvens SDM]
ATGGGCAGAAACCTTGAGCAGCTTATAGCGGATGAAAAATCGGATGTTGTTGCCAGCGCTCAGGCAATGGCTGCCGACAT
TTTGCTTAACATCCATCTGGCAGAGCTACGTGAAAAGGTGCAAAAAACGCAGGTTGAAATGGCGCAGGCGCTGGGTATCC
GACAGCCTACCGTCGCGGTCATGGAAAAGCCCGGGCGCGATCTTAAACTCTCTTCACTCAAGCGCTACGTTGAGGCTGCG
GGTGGAAAGCTTACACTTGACGTTGAGTTACCGGATGGTTCGCATTATGAGTTTGTGCTGTAG

Structures

Toxin

• AlphaFold DB: A0A3R9FZF4