Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 4148963..4149642 Replicon chromosome
Accession NZ_CP124387
Organism Escherichia coli strain AVS0972

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag QJB01_RS19965 Protein ID WP_000057523.1
Coordinates 4148963..4149265 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag QJB01_RS19970 Protein ID WP_000806442.1
Coordinates 4149301..4149642 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QJB01_RS19940 (4144339) 4144339..4145991 + 1653 WP_000771748.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
QJB01_RS19945 (4146029) 4146029..4146532 - 504 WP_000667000.1 hypothetical protein -
QJB01_RS19950 (4146529) 4146529..4147329 - 801 WP_000439798.1 hypothetical protein -
QJB01_RS19955 (4147353) 4147353..4147832 - 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
QJB01_RS19960 (4148036) 4148036..4148830 - 795 WP_000365147.1 TraB/GumN family protein -
QJB01_RS19965 (4148963) 4148963..4149265 + 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
QJB01_RS19970 (4149301) 4149301..4149642 + 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
QJB01_RS19975 (4149700) 4149700..4152204 - 2505 WP_000083947.1 copper-exporting P-type ATPase CopA -
QJB01_RS19980 (4152466) 4152466..4153398 + 933 WP_000883041.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T280001 WP_000057523.1 NZ_CP124387:4148963-4149265 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT280001 WP_000806442.1 NZ_CP124387:4149301-4149642 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References