Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 4359336..4360015 Replicon chromosome
Accession NZ_CP124385
Organism Escherichia coli strain AVS0153

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag QJP67_RS21340 Protein ID WP_000057523.1
Coordinates 4359336..4359638 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag QJP67_RS21345 Protein ID WP_000806442.1
Coordinates 4359674..4360015 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QJP67_RS21315 (4354712) 4354712..4356364 + 1653 WP_000771748.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
QJP67_RS21320 (4356402) 4356402..4356905 - 504 WP_000667000.1 hypothetical protein -
QJP67_RS21325 (4356902) 4356902..4357702 - 801 WP_000439798.1 hypothetical protein -
QJP67_RS21330 (4357726) 4357726..4358205 - 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
QJP67_RS21335 (4358409) 4358409..4359203 - 795 WP_000365147.1 TraB/GumN family protein -
QJP67_RS21340 (4359336) 4359336..4359638 + 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
QJP67_RS21345 (4359674) 4359674..4360015 + 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
QJP67_RS21350 (4360073) 4360073..4362577 - 2505 WP_000083947.1 copper-exporting P-type ATPase CopA -
QJP67_RS21355 (4362839) 4362839..4363771 + 933 WP_000883041.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T279969 WP_000057523.1 NZ_CP124385:4359336-4359638 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT279969 WP_000806442.1 NZ_CP124385:4359674-4360015 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References