Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 1237933..1238612 Replicon chromosome
Accession NZ_CP124374
Organism Escherichia coli strain AVS0578

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag QJP97_RS05945 Protein ID WP_000057523.1
Coordinates 1237933..1238235 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag QJP97_RS05950 Protein ID WP_000806442.1
Coordinates 1238271..1238612 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QJP97_RS05920 (1233309) 1233309..1234961 + 1653 WP_000771748.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
QJP97_RS05925 (1234999) 1234999..1235502 - 504 WP_000667000.1 hypothetical protein -
QJP97_RS05930 (1235499) 1235499..1236155 - 657 WP_015674862.1 hypothetical protein -
QJP97_RS05935 (1236323) 1236323..1236802 - 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
QJP97_RS05940 (1237006) 1237006..1237800 - 795 WP_000365147.1 TraB/GumN family protein -
QJP97_RS05945 (1237933) 1237933..1238235 + 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
QJP97_RS05950 (1238271) 1238271..1238612 + 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
QJP97_RS05955 (1238670) 1238670..1241174 - 2505 WP_000083947.1 copper-exporting P-type ATPase CopA -
QJP97_RS05960 (1241436) 1241436..1242368 + 933 WP_000883041.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T279921 WP_000057523.1 NZ_CP124374:1237933-1238235 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT279921 WP_000806442.1 NZ_CP124374:1238271-1238612 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References