Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 4360914..4361593 Replicon chromosome
Accession NZ_CP124355
Organism Escherichia coli strain AVS0171

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag QJB00_RS21345 Protein ID WP_000057523.1
Coordinates 4360914..4361216 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag QJB00_RS21350 Protein ID WP_000806442.1
Coordinates 4361252..4361593 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QJB00_RS21320 (4356290) 4356290..4357942 + 1653 WP_000771748.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
QJB00_RS21325 (4357980) 4357980..4358483 - 504 WP_000667000.1 hypothetical protein -
QJB00_RS21330 (4358480) 4358480..4359136 - 657 WP_015674862.1 hypothetical protein -
QJB00_RS21335 (4359304) 4359304..4359783 - 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
QJB00_RS21340 (4359987) 4359987..4360781 - 795 WP_000365147.1 TraB/GumN family protein -
QJB00_RS21345 (4360914) 4360914..4361216 + 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
QJB00_RS21350 (4361252) 4361252..4361593 + 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
QJB00_RS21355 (4361651) 4361651..4364155 - 2505 WP_000083947.1 copper-exporting P-type ATPase CopA -
QJB00_RS21360 (4364417) 4364417..4365349 + 933 WP_000883041.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T279791 WP_000057523.1 NZ_CP124355:4360914-4361216 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT279791 WP_000806442.1 NZ_CP124355:4361252-4361593 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References