Detailed information of TA system TA027932

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 1330077..1330687
• Replicon: chromosome
• Accession: NC_018028
• Organism: Pseudomonas stutzeri CCUG 29243

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I4CQU0
• Locus tag: A458_RS27770
• Protein ID: WP_014819565.1
• Coordinates: 1330077..1330391 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: I4CQU1
• Locus tag: A458_RS27775
• Protein ID: WP_003302338.1
• Coordinates: 1330397..1330687 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A458_RS27745	1325779..1326078	+	300	WP_041754657.1	hypothetical protein	-
A458_RS27750	1326078..1326404	+	327	WP_014819561.1	DUF2523 domain-containing protein	-
A458_RS27755	1326417..1327601	+	1185	WP_014819562.1	zonular occludens toxin	-
A458_RS27760	1327748..1329025	+	1278	WP_014819563.1	hypothetical protein	-
A458_RS27765	1329007..1330032	+	1026	WP_014819564.1	tyrosine-type recombinase/integrase	-
A458_RS27770	1330077..1330391	+	315	WP_014819565.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A458_RS27775	1330397..1330687	+	291	WP_003302338.1	helix-turn-helix domain-containing protein	Antitoxin
A458_RS27780	1331054..1331950	-	897	WP_010792197.1	LysR family transcriptional regulator	-
A458_RS27785	1332234..1332842	+	609	WP_014819566.1	TetR/AcrR family transcriptional regulator	-
A458_RS27790	1333027..1333452	+	426	WP_014819567.1	hypothetical protein	-
A458_RS27795	1333480..1334520	+	1041	WP_014819568.1	secretion protein HlyD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1326417..1338583	12166

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (43-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (35-86)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11935.67 Da
• Isoelectric Point: 9.8035

>T27932 WP_014819565.1 NC_018028:1330077-1330391 [Pseudomonas stutzeri CCUG 29243]
MIFIETPIFTKRLRDLLSDDSYAEFQRQLADRPDLGDVIEGTGGIRKVRVASSGHGKRGGSRVIYYHFTAASQIALLLIY
PKNEKDDLTADERKVLKQIIERWR

Nucleotide

• Length: 315 bp

>T27932 NC_018028:1330077-1330391 [Pseudomonas stutzeri CCUG 29243]
ATGATCTTTATCGAGACACCGATCTTCACCAAGCGCCTGCGGGATCTGCTTAGCGATGACAGCTACGCGGAGTTTCAGCG
ACAACTGGCTGACCGGCCGGACTTGGGCGATGTGATTGAAGGTACTGGCGGCATTCGCAAGGTTCGCGTTGCGTCTAGCG
GTCACGGCAAACGAGGCGGGTCTAGGGTCATCTACTACCACTTCACGGCGGCTTCGCAGATCGCACTACTGCTGATCTAT
CCGAAGAACGAGAAGGACGACCTGACGGCAGACGAGCGCAAGGTGCTCAAGCAAATTATCGAGCGGTGGAGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10925.51 Da
• Isoelectric Point: 8.8068

>AT27932 WP_003302338.1 NC_018028:1330397-1330687 [Pseudomonas stutzeri CCUG 29243]
MSKFFEDLLESVQQMDEIHRGERQPSREFVVDSLQVKEIRKATGLTQAKFAAMIDVQLGTLRNWEQGRREPTGPAKALLR
AIHNDPKHVIQALSSV

Nucleotide

• Length: 291 bp

>AT27932 NC_018028:1330397-1330687 [Pseudomonas stutzeri CCUG 29243]
ATGAGCAAATTCTTCGAAGACCTTCTGGAAAGCGTCCAGCAGATGGACGAGATTCACCGTGGCGAGCGTCAGCCCTCGCG
GGAATTCGTCGTCGACTCGCTGCAGGTGAAAGAGATCCGCAAAGCAACCGGTCTGACCCAGGCCAAGTTCGCAGCGATGA
TCGACGTTCAACTGGGTACGCTGCGTAACTGGGAACAAGGTCGACGGGAGCCGACCGGACCGGCAAAGGCGCTGCTGCGC
GCCATCCATAACGATCCGAAGCACGTCATCCAGGCGCTTTCTTCCGTCTGA

Structures

Toxin

• AlphaFold DB: I4CQU0

Antitoxin

• AlphaFold DB: I4CQU1