Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4170112..4170609 Replicon chromosome
Accession NZ_CP123963
Organism Escherichia coli strain L6-21

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag QC817_RS20475 Protein ID WP_001173343.1
Coordinates 4170112..4170285 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag QC817_RS20480 Protein ID WP_000398619.1
Coordinates 4170313..4170609 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QC817_RS20465 (4166782) 4166782..4168239 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
QC817_RS20470 (4168476) 4168476..4169993 + 1518 WP_001295074.1 lysine--tRNA ligase -
QC817_RS20475 (4170112) 4170112..4170285 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
QC817_RS20480 (4170313) 4170313..4170609 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
QC817_RS20485 (4170836) 4170836..4171108 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
QC817_RS20490 (4171120) 4171120..4171350 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
QC817_RS20495 (4171531) 4171531..4173162 + 1632 WP_152949980.1 sensor histidine kinase -
QC817_RS20500 (4173159) 4173159..4173878 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4161258..4170609 9351


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T279288 WP_001173343.1 NZ_CP123963:c4170285-4170112 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT279288 WP_000398619.1 NZ_CP123963:c4170609-4170313 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References