Detailed information of TA system TA027928

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 2605946..2607372
• Replicon: chromosome
• Accession: NC_018025
• Organism: Desulfomonile tiedjei DSM 6799

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I4C5M2
• Locus tag: DESTI_RS10865
• Protein ID: WP_014810006.1
• Coordinates: 2605946..2606281 (+)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: I4C5M3
• Locus tag: DESTI_RS10870
• Protein ID: WP_014810007.1
• Coordinates: 2606281..2607372 (+)
• Length: 364 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DESTI_RS10845	2601348..2602493	-	1146	WP_014810003.1	cation:proton antiporter	-
DESTI_RS28735	2602549..2603850	-	1302	WP_014810004.1	dynamin family protein	-
DESTI_RS10855	2604251..2605519	+	1269	WP_014810005.1	DUF1311 domain-containing protein	-
DESTI_RS10865	2605946..2606281	+	336	WP_014810006.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DESTI_RS10870	2606281..2607372	+	1092	WP_014810007.1	HigA family addiction module antidote protein	Antitoxin
DESTI_RS10875	2607614..2608468	+	855	WP_014810009.1	DUF4238 domain-containing protein	-
DESTI_RS10880	2608542..2608985	-	444	WP_014810010.1	nucleoside 2-deoxyribosyltransferase	-
DESTI_RS10885	2609066..2609641	-	576	WP_014810012.1	hypothetical protein	-
DESTI_RS10890	2609801..2610868	-	1068	WP_014810013.1	hypothetical protein	-
DESTI_RS10895	2611321..2611509	-	189	WP_014810014.1	hypothetical protein	-
DESTI_RS10900	2611937..2612263	+	327	WP_041286133.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-81)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (15-89)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 13125.14 Da
• Isoelectric Point: 10.3545

>T27928 WP_014810006.1 NC_018025:2605946-2606281 [Desulfomonile tiedjei DSM 6799]
MDIDFKSKKLMKIFNSEERLRKEYGPEQARLIQRRMWILRAAPTLADVPTDKPERRHQLKGDRKNQFAVDLKQPFRLIFE
PNHNPIPRSEDGNIITSNVTAITILEVKDYH

Nucleotide

• Length: 336 bp

>T27928 NC_018025:2605946-2606281 [Desulfomonile tiedjei DSM 6799]
ATGGATATAGATTTCAAGTCTAAGAAATTGATGAAGATTTTCAACTCAGAGGAACGTCTTCGCAAAGAATACGGTCCCGA
GCAAGCGCGACTCATCCAAAGGCGGATGTGGATACTTCGCGCCGCACCAACGCTTGCAGATGTACCGACCGATAAGCCAG
AGCGAAGACACCAACTGAAAGGGGACAGGAAAAATCAATTTGCCGTGGATTTGAAACAACCGTTTCGGCTTATTTTTGAG
CCAAACCATAATCCTATTCCGAGGTCAGAAGACGGTAATATCATCACCAGTAACGTCACTGCGATTACGATACTGGAAGT
AAAGGACTATCACTGA

Antitoxin

Protein

• Length: 364 a.a.
• Molecular weight: 41503.56 Da
• Isoelectric Point: 9.3778

>AT27928 WP_014810007.1 NC_018025:2606281-2607372 [Desulfomonile tiedjei DSM 6799]
MITQTANKFTPDYVISPGEILEETLEARGIKKKEFAERCGLSDKTVSLIIHGKAPVLPETAIQFERVLGVSAAIWNNLEA
AYRLHHANKAEQDKLNTQRQWAKRFPVNELAKRGFIEKPDDAIDAVRKLLNFFAVASIEIWEKRYASMLVAYRHSPSFKS
APEAVATWLRIGELVAERIETAPYSRDTFLAALNQIRSLTSSSDFEHKMKTLCQEAGIALVFVPEFKNTRLSGATRWLTK
DKALIILSLRHKRDDHLWFSFFHESAHILHHGKKETFLDEKDTPESDQEKEADAFAADFLIPAKSYRDFIKNKIFSEKSI
RLFAEQQGIAPGIIVGRLQRDKQITYGSLNHLKRVFEFKTEIS

Nucleotide

• Length: 1092 bp

>AT27928 NC_018025:2606281-2607372 [Desulfomonile tiedjei DSM 6799]
ATGATCACACAAACAGCCAATAAATTTACTCCGGATTATGTCATATCACCTGGAGAAATTCTTGAGGAAACCCTCGAGGC
GCGGGGGATCAAAAAGAAAGAATTCGCCGAACGATGCGGCCTCTCGGATAAGACTGTAAGTCTCATAATTCACGGGAAAG
CTCCAGTTCTACCAGAAACAGCTATCCAGTTTGAGCGGGTGCTGGGGGTCTCCGCGGCAATATGGAACAACCTTGAAGCC
GCGTATCGTTTACACCATGCCAACAAAGCCGAACAAGATAAACTCAATACTCAGCGGCAATGGGCGAAACGTTTCCCTGT
CAATGAGCTCGCCAAAAGAGGGTTTATAGAAAAGCCGGACGATGCAATTGATGCCGTCAGGAAGCTCTTGAATTTCTTCG
CTGTCGCGTCAATAGAAATTTGGGAGAAGCGCTATGCTTCCATGCTTGTGGCATATAGGCATTCTCCGTCCTTCAAGAGC
GCACCCGAAGCTGTAGCTACCTGGTTGAGAATAGGGGAACTGGTGGCAGAAAGAATAGAAACTGCACCATATTCCAGAGA
TACCTTTCTTGCTGCCTTGAATCAGATAAGAAGTTTAACAAGCTCATCCGATTTTGAACATAAAATGAAGACACTTTGCC
AAGAAGCCGGCATAGCATTAGTTTTTGTGCCAGAATTTAAGAATACGCGTCTCAGTGGCGCAACACGTTGGCTCACAAAG
GACAAAGCGCTGATCATCCTCAGTCTTCGCCACAAAAGAGATGATCATTTGTGGTTCAGTTTCTTTCACGAGTCTGCCCA
CATTTTGCACCACGGGAAAAAAGAGACATTTCTGGACGAGAAAGACACACCTGAAAGTGATCAAGAAAAAGAAGCGGATG
CTTTTGCAGCGGACTTCTTGATACCTGCCAAGAGTTATCGTGATTTCATAAAGAATAAAATCTTTTCCGAAAAATCAATC
AGGTTATTTGCTGAACAGCAAGGCATAGCACCTGGAATAATCGTTGGGAGACTCCAACGAGACAAACAGATTACATATGG
TAGCCTCAATCATCTAAAAAGAGTCTTTGAATTCAAGACAGAAATTTCATAA

Structures

Toxin

• AlphaFold DB: I4C5M2

Antitoxin

• AlphaFold DB: I4C5M3