Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 299410..300104 Replicon chromosome
Accession NZ_CP123365
Organism Shigella flexneri strain MMGSG_23

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag PFB08_RS01455 Protein ID WP_001263491.1
Coordinates 299706..300104 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID Q0T7Q4
Locus tag PFB08_RS01450 Protein ID WP_000554759.1
Coordinates 299410..299703 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PFB08_RS01430 (295049) 295049..295546 + 498 WP_000006251.1 REP-associated tyrosine transposase RayT -
PFB08_RS01435 (295763) 295763..297475 - 1713 Protein_278 flagellar biosynthesis protein FlhA -
PFB08_RS01440 (297447) 297447..298232 + 786 WP_000207560.1 putative lateral flagellar export/assembly protein LafU -
PFB08_RS01445 (298303) 298303..299358 + 1056 WP_001226172.1 DNA polymerase IV -
PFB08_RS01450 (299410) 299410..299703 + 294 WP_000554759.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
PFB08_RS01455 (299706) 299706..300104 + 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
PFB08_RS01460 (300114) 300114..300566 + 453 WP_001059860.1 GNAT family N-acetyltransferase -
PFB08_RS01465 (300812) 300812..301855 + 1044 WP_005053205.1 RNA ligase RtcB family protein -
PFB08_RS01470 (301917) 301917..302531 + 615 Protein_285 peptide chain release factor H -
PFB08_RS01475 (302588) 302588..304045 - 1458 WP_001293030.1 cytosol nonspecific dipeptidase -
PFB08_RS01480 (304307) 304307..304765 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - gtrA / gtrB / gtrII 298303..339701 41398


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T278942 WP_001263491.1 NZ_CP123365:299706-300104 [Shigella flexneri]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11232.82 Da        Isoelectric Point: 7.0153

>AT278942 WP_000554759.1 NZ_CP123365:299410-299703 [Shigella flexneri]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
KQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TR46

References