Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 39375..39910 Replicon plasmid 3
Accession NC_017963
Organism Enterococcus faecium DO

Toxin (Protein)


Gene name yoeB Uniprot ID Q3Y221
Locus tag HMPREF0351_RS13845 Protein ID WP_002305108.1
Coordinates 39638..39910 (+) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID Q3Y220
Locus tag HMPREF0351_RS13840 Protein ID WP_002297422.1
Coordinates 39375..39638 (+) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HMPREF0351_RS15030 34738..34824 + 87 Protein_32 single-stranded DNA-binding protein -
HMPREF0351_RS13815 35250..36428 - 1179 WP_000997695.1 IS256-like element ISEf1 family transposase -
HMPREF0351_RS13820 36730..36948 + 219 Protein_34 type II toxin-antitoxin system RelE/ParE family toxin -
HMPREF0351_RS13825 37208..37462 + 255 WP_002290397.1 AbrB family transcriptional regulator -
HMPREF0351_RS13830 37463..37780 + 318 WP_002290394.1 type II toxin-antitoxin system RelE/ParE family toxin -
HMPREF0351_RS13835 38339..39265 + 927 WP_002390052.1 hypothetical protein -
HMPREF0351_RS13840 39375..39638 + 264 WP_002297422.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
HMPREF0351_RS13845 39638..39910 + 273 WP_002305108.1 Txe/YoeB family addiction module toxin Toxin
HMPREF0351_RS13850 39987..40199 + 213 WP_002390051.1 hypothetical protein -
HMPREF0351_RS13855 40212..40892 - 681 WP_002390049.1 IS6 family transposase -
HMPREF0351_RS13860 40967..41179 + 213 Protein_42 hypothetical protein -
HMPREF0351_RS13865 41592..42335 - 744 WP_002390046.1 IS21-like element helper ATPase IstB -
HMPREF0351_RS13870 42335..43759 - 1425 WP_002390044.1 IS21 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..251926 251926
- inside IScluster/Tn - - 35250..40892 5642


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(9-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-69)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10806.28 Da        Isoelectric Point: 9.9080

>T27886 WP_002305108.1 NC_017963:39638-39910 [Enterococcus faecium DO]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTYEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Download         Length: 273 bp

>T27886 NC_017963:39638-39910 [Enterococcus faecium DO]
ATGAATAACTATTCTGTGATGATTAAAAACTCGGCTAAAGCTGATTTAAAGAAAATCAAACAGTCTAATTTGAAACCTCA
ATTTGAGAAGGTTATTCAGACTTTAAAGGAAGATCCCTATCTCCCTACCCAATCATTTGAGAAGTTAAAACCTACATATG
AAGGTAGGTATTCTAGAAGATTGAATAGACAACACCGGGTTGTTTATAAGGTAGATGAAGATGAACACGTGGTTGAAATT
TATTCAGCAAGGACGCATTACGAGTCACAGTAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9959.22 Da        Isoelectric Point: 4.5370

>AT27886 WP_002297422.1 NC_017963:39375-39638 [Enterococcus faecium DO]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Download         Length: 264 bp

>AT27886 NC_017963:39375-39638 [Enterococcus faecium DO]
ATGGCAAAAACAGTCTTAAATCCTAGCTCAGCACGGAAAAATTTTTATAAGTTGTTGAAAGATGTAAATGAGAATCATAC
GGAAATTGAAATCATTAGTGAGCGCAGTGAAAATAATGCGGTGCTTATTGGTTTGGATGATTGGAAAGCAATCAAAGAAA
CGCTATTACTTGAGCAAATAGGCACTTTGGGTGTTGTTCGTAAGCGAGAAAAAGATGATAGTGGATTTACTAATGTTGAT
GAAATTGATTGGGATAACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A133CER3

Antitoxin

Source ID Structure
AlphaFold DB A0A829F006

References