Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3819813..3820507 Replicon chromosome
Accession NZ_CP123255
Organism Escherichia coli strain YZMc17-1

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag N4680_RS18545 Protein ID WP_001263489.1
Coordinates 3819813..3820211 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag N4680_RS18550 Protein ID WP_000554758.1
Coordinates 3820214..3820507 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3815401) 3815401..3815481 - 81 NuclAT_12 - -
- (3815401) 3815401..3815481 - 81 NuclAT_12 - -
- (3815401) 3815401..3815481 - 81 NuclAT_12 - -
- (3815401) 3815401..3815481 - 81 NuclAT_12 - -
N4680_RS18520 (3816077) 3816077..3816535 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
N4680_RS18525 (3816796) 3816796..3818253 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
N4680_RS18530 (3818310) 3818310..3818831 - 522 Protein_3626 peptide chain release factor H -
N4680_RS18535 (3818827) 3818827..3819033 - 207 Protein_3627 RtcB family protein -
N4680_RS18540 (3819351) 3819351..3819803 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
N4680_RS18545 (3819813) 3819813..3820211 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
N4680_RS18550 (3820214) 3820214..3820507 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
N4680_RS18555 (3820559) 3820559..3821614 - 1056 WP_001226164.1 DNA polymerase IV -
N4680_RS18560 (3821685) 3821685..3822470 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
N4680_RS18565 (3822442) 3822442..3824154 + 1713 Protein_3633 flagellar biosynthesis protein FlhA -
N4680_RS18570 (3824378) 3824378..3824875 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T278809 WP_001263489.1 NZ_CP123255:c3820211-3819813 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT278809 WP_000554758.1 NZ_CP123255:c3820507-3820214 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References