Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1119204..1119931 Replicon chromosome
Accession NZ_CP123255
Organism Escherichia coli strain YZMc17-1

Toxin (Protein)


Gene name higB Uniprot ID A0A829L2T9
Locus tag N4680_RS05490 Protein ID WP_001521139.1
Coordinates 1119204..1119515 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag N4680_RS05495 Protein ID WP_000126294.1
Coordinates 1119512..1119931 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N4680_RS05465 (1115139) 1115139..1116848 + 1710 WP_001521140.1 formate hydrogenlyase subunit HycE -
N4680_RS05470 (1116858) 1116858..1117400 + 543 WP_000493785.1 formate hydrogenlyase subunit HycF -
N4680_RS05475 (1117400) 1117400..1118167 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
N4680_RS05480 (1118164) 1118164..1118574 + 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
N4680_RS05485 (1118567) 1118567..1119037 + 471 WP_000132961.1 hydrogenase maturation peptidase HycI -
N4680_RS05490 (1119204) 1119204..1119515 + 312 WP_001521139.1 type II toxin-antitoxin system HigB family toxin Toxin
N4680_RS05495 (1119512) 1119512..1119931 + 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
N4680_RS05500 (1120010) 1120010..1121434 - 1425 WP_020234028.1 6-phospho-beta-glucosidase AscB -
N4680_RS05505 (1121443) 1121443..1122900 - 1458 WP_020234027.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
N4680_RS05510 (1123160) 1123160..1124170 + 1011 WP_015912547.1 DNA-binding transcriptional regulator AscG -
N4680_RS05515 (1124319) 1124319..1124846 + 528 WP_001078777.1 electron transport protein HydN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12493.23 Da        Isoelectric Point: 9.4783

>T278800 WP_001521139.1 NZ_CP123255:1119204-1119515 [Escherichia coli]
MHIISKAPFEECARKYPNDALALYSLYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT278800 WP_000126294.1 NZ_CP123255:1119512-1119931 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829L2T9

Antitoxin

Source ID Structure

References