Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 5014743..5015345 Replicon chromosome
Accession NZ_CP123240
Organism Escherichia coli strain YZLc1-3

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag N4725_RS24275 Protein ID WP_000897305.1
Coordinates 5015034..5015345 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag N4725_RS24270 Protein ID WP_000356395.1
Coordinates 5014743..5015033 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N4725_RS24235 (5010367) 5010367..5011269 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
N4725_RS24240 (5011266) 5011266..5011901 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
N4725_RS24245 (5011898) 5011898..5012827 + 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
N4725_RS24250 (5013009) 5013009..5013251 - 243 WP_001309881.1 CopG family transcriptional regulator -
N4725_RS24255 (5013470) 5013470..5013688 - 219 WP_001295676.1 CopG family transcriptional regulator -
N4725_RS24260 (5014107) 5014107..5014385 - 279 WP_001315112.1 hypothetical protein -
N4725_RS24265 (5014437) 5014437..5014658 - 222 WP_106475282.1 cobalt ABC transporter ATP-binding protein -
N4725_RS24270 (5014743) 5014743..5015033 - 291 WP_000356395.1 NadS family protein Antitoxin
N4725_RS24275 (5015034) 5015034..5015345 - 312 WP_000897305.1 hypothetical protein Toxin
N4725_RS24280 (5015574) 5015574..5016482 + 909 WP_001550353.1 alpha/beta hydrolase -
N4725_RS24285 (5016650) 5016650..5017564 - 915 WP_109553727.1 transposase -
N4725_RS24290 (5017577) 5017577..5018464 - 888 Protein_4749 hypothetical protein -
N4725_RS24295 (5018880) 5018880..5019821 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
N4725_RS24300 (5019866) 5019866..5020303 - 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T278749 WP_000897305.1 NZ_CP123240:c5015345-5015034 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10869.46 Da        Isoelectric Point: 10.8907

>AT278749 WP_000356395.1 NZ_CP123240:c5015033-5014743 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References