Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1258176..1258903 Replicon chromosome
Accession NZ_CP123240
Organism Escherichia coli strain YZLc1-3

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag N4725_RS06150 Protein ID WP_000547555.1
Coordinates 1258176..1258487 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag N4725_RS06155 Protein ID WP_000126294.1
Coordinates 1258484..1258903 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N4725_RS06125 (1254111) 1254111..1255820 + 1710 WP_001288122.1 formate hydrogenlyase subunit HycE -
N4725_RS06130 (1255830) 1255830..1256372 + 543 WP_000493785.1 formate hydrogenlyase subunit HycF -
N4725_RS06135 (1256372) 1256372..1257139 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
N4725_RS06140 (1257136) 1257136..1257546 + 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
N4725_RS06145 (1257539) 1257539..1258009 + 471 WP_000132961.1 hydrogenase maturation peptidase HycI -
N4725_RS06150 (1258176) 1258176..1258487 + 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
N4725_RS06155 (1258484) 1258484..1258903 + 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
N4725_RS06160 (1258982) 1258982..1260406 - 1425 WP_000110355.1 6-phospho-beta-glucosidase AscB -
N4725_RS06165 (1260415) 1260415..1261872 - 1458 WP_001107889.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
N4725_RS06170 (1262132) 1262132..1263142 + 1011 WP_029130985.1 DNA-binding transcriptional regulator AscG -
N4725_RS06175 (1263291) 1263291..1263818 + 528 WP_001078777.1 electron transport protein HydN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T278731 WP_000547555.1 NZ_CP123240:1258176-1258487 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT278731 WP_000126294.1 NZ_CP123240:1258484-1258903 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure

References