Detailed information of TA system TA027873

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1851660..1852281
• Replicon: chromosome
• Accession: NC_017956
• Organism: Tistrella mobilis KA081020-065

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: TMO_RS08390
• Protein ID: WP_041604877.1
• Coordinates: 1851660..1851938 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: TMO_RS08395
• Protein ID: WP_014745187.1
• Coordinates: 1851988..1852281 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TMO_RS08370	1847246..1848202	-	957	WP_014745183.1	ABC transporter substrate-binding protein	-
TMO_RS08375	1848488..1848844	-	357	WP_014745184.1	DUF1304 domain-containing protein	-
TMO_RS08380	1849103..1850494	+	1392	WP_014745185.1	diguanylate cyclase	-
TMO_RS08385	1850499..1851479	-	981	WP_014745186.1	alpha/beta hydrolase	-
TMO_RS08390	1851660..1851938	+	279	WP_041604877.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
TMO_RS08395	1851988..1852281	+	294	WP_014745187.1	HigA family addiction module antidote protein	Antitoxin
TMO_RS30325	1852478..1853646	+	1169	Protein_1690	IS256 family transposase	-
TMO_RS08420	1855457..1856512	+	1056	WP_145978216.1	lytic murein transglycosylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (23-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10762.24 Da
• Isoelectric Point: 8.5613

>T27873 WP_041604877.1 NC_017956:1851660-1851938 [Tistrella mobilis KA081020-065]
MIQSFACRETEALFNRERSRRFGAIERQARRKLLMLDAAHVLDDLRSPPGNRLEALRGDRAGAHSIRINDQWRLCFIWTE
AGPRDVEIVDYH

Nucleotide

• Length: 279 bp

>T27873 NC_017956:1851660-1851938 [Tistrella mobilis KA081020-065]
ATGATCCAATCTTTTGCGTGCCGGGAAACGGAAGCCCTCTTCAATCGGGAACGATCCCGACGGTTCGGCGCCATTGAACG
TCAGGCCCGCCGCAAACTTCTGATGCTCGACGCAGCCCACGTTCTCGATGATCTGCGCAGCCCTCCCGGTAATCGCTTGG
AAGCCCTTCGCGGCGACCGGGCGGGTGCGCACTCGATCCGGATCAATGATCAGTGGCGACTGTGCTTCATCTGGACAGAA
GCCGGCCCCCGAGACGTCGAAATTGTGGACTACCATTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10680.38 Da
• Isoelectric Point: 4.7005

>AT27873 WP_014745187.1 NC_017956:1851988-1852281 [Tistrella mobilis KA081020-065]
MPTKYEPIHPGEILSTEFLDPLGVSATELARAIHVPPNRVTRLINGQAAVTPDTALRLARALRTTPEFWLNLQMRFDLDM
AADAGGVMDDIQPIVVM

Nucleotide

• Length: 294 bp

>AT27873 NC_017956:1851988-1852281 [Tistrella mobilis KA081020-065]
ATGCCGACAAAATACGAGCCCATTCACCCCGGTGAGATCCTGAGCACCGAATTCCTCGACCCCCTCGGTGTCTCCGCAAC
GGAACTCGCACGCGCCATCCACGTGCCCCCTAATCGCGTGACCCGTCTGATCAACGGACAGGCCGCGGTGACTCCTGACA
CCGCGCTGCGACTGGCCCGCGCCCTGCGCACGACGCCGGAATTCTGGCTGAATTTGCAGATGCGGTTTGATCTGGATATG
GCCGCAGATGCGGGTGGAGTGATGGATGATATTCAACCGATTGTCGTTATGTAA