Detailed information of TA system TA027871

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 352301..352988
• Replicon: chromosome
• Accession: NC_017956
• Organism: Tistrella mobilis KA081020-065

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I3THD5
• Locus tag: TMO_RS01710
• Protein ID: WP_014743853.1
• Coordinates: 352629..352988 (-)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: TMO_RS01705
• Protein ID: WP_051045286.1
• Coordinates: 352301..352624 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TMO_RS01680	347716..348588	+	873	WP_014743846.1	branched-chain amino acid ABC transporter permease	-
TMO_RS01685	348601..349506	+	906	WP_014743847.1	branched-chain amino acid ABC transporter permease	-
TMO_RS01690	349511..350320	+	810	WP_014743848.1	ABC transporter ATP-binding protein	-
TMO_RS01695	350317..351021	+	705	WP_014743849.1	ABC transporter ATP-binding protein	-
TMO_RS01700	351035..352294	+	1260	WP_014743850.1	amidohydrolase family protein	-
TMO_RS01705	352301..352624	-	324	WP_051045286.1	XRE family transcriptional regulator	Antitoxin
TMO_RS01710	352629..352988	-	360	WP_014743853.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
TMO_RS01715	353188..355068	-	1881	WP_014743854.1	cobaltochelatase subunit CobT	-
TMO_RS01720	355074..356063	-	990	WP_041605292.1	cobaltochelatase subunit CobS	-
TMO_RS01725	356229..356840	-	612	WP_014743856.1	J domain-containing protein	-
TMO_RS01730	357025..357393	-	369	WP_014743857.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-108)

Antitoxin

• HigA2-like, helix-turn-helix domain (9-89)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13629.64 Da
• Isoelectric Point: 9.5025

>T27871 WP_014743853.1 NC_017956:c352988-352629 [Tistrella mobilis KA081020-065]
MPWQVIFDDDFVEEYEMLPHDLRLAILSGARLLSEYGPGLGRPYVDTLRGSAFPNMKELRLRSTDGAWRIAFAFDPRRQA
VLLIAGNKAGKSQKRFYTRLITIADARFTDHLARTSLQE

Nucleotide

• Length: 360 bp

>T27871 NC_017956:c352988-352629 [Tistrella mobilis KA081020-065]
ATGCCGTGGCAGGTGATCTTCGATGACGACTTCGTGGAAGAATACGAGATGCTGCCGCACGACCTGCGGCTGGCAATACT
GTCGGGAGCACGACTGCTGTCGGAATACGGACCGGGCCTGGGACGCCCCTATGTCGATACGCTCCGGGGATCGGCCTTCC
CCAATATGAAGGAGTTACGCCTGAGATCGACTGATGGCGCATGGCGTATCGCCTTTGCCTTCGATCCCCGACGACAGGCT
GTCTTGCTGATTGCCGGCAACAAGGCCGGAAAGAGCCAGAAACGTTTTTACACCCGCCTCATCACCATCGCCGATGCACG
TTTCACCGATCACCTTGCACGAACATCACTTCAGGAATGA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11697.40 Da
• Isoelectric Point: 4.6677

>AT27871 WP_051045286.1 NC_017956:c352624-352301 [Tistrella mobilis KA081020-065]
MARTLEDMMAALPPEDQAKVDAGFQEMKGEVESLRALRQIAGKAQADVAAALHIRQPSVSRIERQADMYLSTLRNYIEAI
GGELELTVRLPDQPPLRLQGLGDISPG

Nucleotide

• Length: 324 bp

>AT27871 NC_017956:c352624-352301 [Tistrella mobilis KA081020-065]
ATGGCACGCACCCTTGAAGACATGATGGCCGCACTCCCTCCAGAGGATCAGGCAAAGGTTGATGCCGGTTTTCAGGAGAT
GAAGGGCGAGGTCGAGAGCCTCCGCGCCCTCCGTCAGATCGCGGGCAAAGCCCAGGCGGATGTCGCGGCGGCCCTTCACA
TCCGCCAGCCATCGGTCTCGCGGATCGAGAGGCAGGCAGACATGTACCTCTCGACCCTTCGCAACTATATCGAAGCAATC
GGGGGCGAACTGGAGCTGACCGTCCGCCTGCCCGATCAGCCGCCTTTGCGCCTGCAGGGACTGGGAGATATCAGCCCCGG
CTGA

Structures

Toxin

• AlphaFold DB: I3THD5