Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3734941..3735635 Replicon chromosome
Accession NZ_CP123231
Organism Escherichia coli strain 5132

Toxin (Protein)


Gene name yafO Uniprot ID S1PJQ2
Locus tag QET41_RS18235 Protein ID WP_001263500.1
Coordinates 3734941..3735339 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag QET41_RS18240 Protein ID WP_000554758.1
Coordinates 3735342..3735635 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QET41_RS18210 (3730306) 3730306..3730764 - 459 WP_001291991.1 xanthine phosphoribosyltransferase -
QET41_RS18215 (3731025) 3731025..3732482 + 1458 WP_001292999.1 cytosol nonspecific dipeptidase -
QET41_RS18220 (3732539) 3732539..3733153 - 615 WP_000602124.1 peptide chain release factor H -
QET41_RS18225 (3733150) 3733150..3734289 - 1140 WP_000521561.1 RNA ligase RtcB family protein -
QET41_RS18230 (3734479) 3734479..3734931 - 453 WP_001059895.1 GNAT family N-acetyltransferase -
QET41_RS18235 (3734941) 3734941..3735339 - 399 WP_001263500.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
QET41_RS18240 (3735342) 3735342..3735635 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
QET41_RS18245 (3735687) 3735687..3736742 - 1056 WP_001226168.1 DNA polymerase IV -
QET41_RS18250 (3736813) 3736813..3737736 - 924 WP_001532973.1 putative lateral flagellar export/assembly protein LafU -
QET41_RS18255 (3737739) 3737739..3738602 - 864 WP_001169518.1 flagellar motor stator protein MotA -
QET41_RS18260 (3738615) 3738615..3739331 - 717 WP_000938731.1 FliA/WhiG family RNA polymerase sigma factor -
QET41_RS18265 (3739351) 3739351..3739818 - 468 WP_000725261.1 flagellar basal body-associated FliL family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15413.85 Da        Isoelectric Point: 8.9511

>T278683 WP_001263500.1 NZ_CP123231:c3735339-3734941 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT278683 WP_000554758.1 NZ_CP123231:c3735635-3735342 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PJQ2

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References