Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3507202..3507881 Replicon chromosome
Accession NZ_CP123231
Organism Escherichia coli strain 5132

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag QET41_RS17185 Protein ID WP_000057523.1
Coordinates 3507579..3507881 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag QET41_RS17180 Protein ID WP_000806442.1
Coordinates 3507202..3507543 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QET41_RS17170 (3503446) 3503446..3504378 - 933 WP_001538399.1 glutaminase A -
QET41_RS17175 (3504640) 3504640..3507144 + 2505 WP_000083948.1 copper-exporting P-type ATPase CopA -
QET41_RS17180 (3507202) 3507202..3507543 - 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
QET41_RS17185 (3507579) 3507579..3507881 - 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
QET41_RS17190 (3508014) 3508014..3508808 + 795 WP_001538398.1 TraB/GumN family protein -
QET41_RS17195 (3509012) 3509012..3509491 + 480 WP_001538397.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
QET41_RS17200 (3509528) 3509528..3511180 - 1653 WP_001773867.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
QET41_RS17205 (3511398) 3511398..3512618 + 1221 WP_001773866.1 fosmidomycin MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T278681 WP_000057523.1 NZ_CP123231:c3507881-3507579 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT278681 WP_000806442.1 NZ_CP123231:c3507543-3507202 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References