Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2465926..2466488 Replicon chromosome
Accession NZ_CP123231
Organism Escherichia coli strain 5132

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag QET41_RS11900 Protein ID WP_000605675.1
Coordinates 2465926..2466204 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag QET41_RS11905 Protein ID WP_000781370.1
Coordinates 2466204..2466488 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QET41_RS11875 (2461512) 2461512..2461943 - 432 WP_000152305.1 peroxiredoxin OsmC -
QET41_RS11880 (2462288) 2462288..2462503 + 216 WP_000495766.1 biofilm-dependent modulation protein -
QET41_RS11885 (2462605) 2462605..2462742 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
QET41_RS11890 (2462899) 2462899..2464596 + 1698 WP_000433460.1 malate dehydrogenase -
QET41_RS11895 (2464730) 2464730..2465740 + 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
QET41_RS11900 (2465926) 2465926..2466204 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
QET41_RS11905 (2466204) 2466204..2466488 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
QET41_RS11910 (2466717) 2466717..2467370 - 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
QET41_RS11915 (2467363) 2467363..2468247 - 885 WP_001240584.1 formate dehydrogenase N subunit beta -
QET41_RS11920 (2468260) 2468260..2471307 - 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T278680 WP_000605675.1 NZ_CP123231:2465926-2466204 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T278680 NZ_CP123231:2465926-2466204 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT278680 WP_000781370.1 NZ_CP123231:2466204-2466488 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT278680 NZ_CP123231:2466204-2466488 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References