Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 141852..142651 Replicon chromosome
Accession NZ_CP123197
Organism Escherichia coli strain C-SRM-3

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag PAE60_RS01855 Protein ID WP_000347251.1
Coordinates 141852..142316 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag PAE60_RS01860 Protein ID WP_001307405.1
Coordinates 142316..142651 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PAE60_RS01825 (136853) 136853..137287 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
PAE60_RS01830 (137305) 137305..138183 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
PAE60_RS01835 (138173) 138173..138952 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
PAE60_RS01840 (138963) 138963..139436 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
PAE60_RS01845 (139459) 139459..140739 - 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
PAE60_RS01850 (140988) 140988..141797 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
PAE60_RS01855 (141852) 141852..142316 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
PAE60_RS01860 (142316) 142316..142651 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
PAE60_RS01865 (142800) 142800..144371 - 1572 WP_001273761.1 galactarate dehydratase -
PAE60_RS01870 (144746) 144746..146080 + 1335 WP_000599652.1 galactarate/glucarate/glycerate transporter GarP -
PAE60_RS01875 (146096) 146096..146866 + 771 WP_001058214.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T278616 WP_000347251.1 NZ_CP123197:c142316-141852 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT278616 WP_001307405.1 NZ_CP123197:c142651-142316 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References