Detailed information of TA system TA027859

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2170902..2171467
• Replicon: chromosome
• Accession: NC_017933
• Organism: Cronobacter sakazakii ES15

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A3Q9CIP0
• Locus tag: ES15_RS10250
• Protein ID: WP_012124820.1
• Coordinates: 2171189..2171467 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: ES15_RS10245
• Protein ID: WP_004388462.1
• Coordinates: 2170902..2171189 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ES15_RS10230	2166499..2167365	+	867	WP_014728910.1	ABC transporter permease	-
ES15_RS10235	2167395..2168453	-	1059	WP_014728911.1	YncE family protein	-
ES15_RS10240	2168730..2170847	+	2118	WP_014728912.1	TonB-dependent receptor	-
ES15_RS10245	2170902..2171189	-	288	WP_004388462.1	HigA family addiction module antidote protein	Antitoxin
ES15_RS10250	2171189..2171467	-	279	WP_012124820.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ES15_RS10255	2171766..2173370	+	1605	WP_014728913.1	alpha-amylase family protein	-
ES15_RS10260	2173372..2174358	-	987	WP_014728914.1	SDR family oxidoreductase	-
ES15_RS10265	2174466..2176403	-	1938	WP_014728915.1	HAMP domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (11-65)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10628.13 Da
• Isoelectric Point: 10.4224

>T27859 WP_012124820.1 NC_017933:c2171467-2171189 [Cronobacter sakazakii ES15]
MIRSFRHKGLQRYFETGSTAGIHARHARKISLRLAVLNQAIRPADVDLPGFFLHPLTGDRKGIWAVTVSGNWRITFEFRD
GDVFIVNYEDYH

Nucleotide

• Length: 279 bp

>T27859 NC_017933:c2171467-2171189 [Cronobacter sakazakii ES15]
ATGATCAGAAGCTTCAGGCATAAAGGCCTGCAACGCTATTTCGAAACGGGTTCCACCGCAGGCATTCATGCCCGCCATGC
CAGAAAGATTTCTCTGCGGCTGGCGGTGCTGAATCAGGCGATAAGACCCGCTGACGTCGACTTACCGGGCTTTTTCCTGC
ATCCCCTGACGGGGGATCGTAAAGGTATATGGGCTGTGACCGTCAGCGGAAACTGGCGCATCACGTTTGAGTTTCGGGAT
GGCGATGTCTTTATCGTCAACTATGAGGATTACCACTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10428.03 Da
• Isoelectric Point: 8.7791

>AT27859 WP_004388462.1 NC_017933:c2171189-2170902 [Cronobacter sakazakii ES15]
MATMFNPPHPGRLVQESMEALGLSARALAKALDVAPSTVQRLLVGKSDVSPEMALRLSAVLGSSPHVWLGMQNEYDLFQA
RQSVDFSHLQRLQRA

Nucleotide

• Length: 288 bp

>AT27859 NC_017933:c2171189-2170902 [Cronobacter sakazakii ES15]
ATGGCAACGATGTTTAACCCACCGCACCCGGGCAGGCTGGTGCAGGAGTCAATGGAAGCGCTCGGCCTCAGCGCCCGCGC
GCTGGCCAAAGCGCTGGACGTCGCGCCGTCGACGGTGCAGCGTCTGCTGGTCGGCAAATCTGACGTCTCCCCGGAAATGG
CGCTGCGTCTCTCGGCAGTCCTTGGCAGCTCGCCGCATGTCTGGCTGGGTATGCAGAACGAATACGATCTCTTTCAGGCG
CGCCAGTCTGTTGATTTCTCGCACCTGCAACGGTTGCAGAGGGCATAA

Structures

Toxin

• AlphaFold DB: A0A3Q9CIP0