Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1114257..1114984 Replicon chromosome
Accession NZ_CP123017
Organism Escherichia coli strain 19-7

Toxin (Protein)


Gene name higB Uniprot ID J7Q991
Locus tag QEN55_RS05470 Protein ID WP_000547564.1
Coordinates 1114257..1114568 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag QEN55_RS05475 Protein ID WP_000126294.1
Coordinates 1114565..1114984 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QEN55_RS05440 (1109399) 1109399..1111108 + 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -
QEN55_RS05445 (1111118) 1111118..1111660 + 543 WP_000493792.1 formate hydrogenlyase subunit HycF -
QEN55_RS05450 (1111660) 1111660..1112427 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
QEN55_RS05455 (1112424) 1112424..1112834 + 411 WP_001291921.1 formate hydrogenlyase assembly protein HycH -
QEN55_RS05460 (1112827) 1112827..1113297 + 471 WP_060621146.1 hydrogenase maturation peptidase HycI -
QEN55_RS05465 (1113322) 1113322..1114083 + 762 WP_001026446.1 hypothetical protein -
QEN55_RS05470 (1114257) 1114257..1114568 + 312 WP_000547564.1 type II toxin-antitoxin system HigB family toxin Toxin
QEN55_RS05475 (1114565) 1114565..1114984 + 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
QEN55_RS05480 (1115098) 1115098..1116522 - 1425 WP_000110363.1 6-phospho-beta-glucosidase AscB -
QEN55_RS05485 (1116531) 1116531..1117988 - 1458 WP_001107861.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
QEN55_RS05490 (1118248) 1118248..1119258 + 1011 WP_001392554.1 DNA-binding transcriptional regulator AscG -
QEN55_RS05495 (1119407) 1119407..1119934 + 528 WP_001078777.1 electron transport protein HydN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12426.13 Da        Isoelectric Point: 9.7248

>T278440 WP_000547564.1 NZ_CP123017:1114257-1114568 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT278440 WP_000126294.1 NZ_CP123017:1114565-1114984 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FJG5

Antitoxin

Source ID Structure

References