Detailed information of TA system TA027843

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-couple_hipB
• Location: 668405..669053
• Replicon: chromosome
• Accession: NC_017927
• Organism: Streptococcus thermophilus MN-ZLW-002

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: Y1U_RS03550
• Protein ID: WP_014727406.1
• Coordinates: 668405..668770 (+)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: J7TXL7
• Locus tag: Y1U_RS03555
• Protein ID: WP_002890617.1
• Coordinates: 668760..669053 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Y1U_RS03535	664912..666057	+	1146	WP_014727403.1	abortive infection protein	-
Y1U_RS03540	666219..666938	+	720	WP_014727404.1	hypothetical protein	-
Y1U_RS10760	666990..668240	+	1251	WP_014727405.1	restriction endonuclease subunit S	-
Y1U_RS03550	668405..668770	+	366	WP_014727406.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
Y1U_RS03555	668760..669053	+	294	WP_002890617.1	helix-turn-helix transcriptional regulator	Antitoxin
Y1U_RS03560	669075..670676	+	1602	WP_041829288.1	type I restriction-modification system subunit M	-
Y1U_RS03565	670754..671173	+	420	Protein_650	PD-(D/E)XK nuclease family transposase	-
Y1U_RS03570	671274..672224	+	951	Protein_651	DNA/RNA non-specific endonuclease	-
Y1U_RS03575	672424..672984	+	561	WP_014621411.1	LemA family protein	-
Y1U_RS03580	672986..673885	+	900	WP_014621412.1	zinc metalloprotease HtpX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (36-109)

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-88)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 14511.65 Da
• Isoelectric Point: 9.3397

>T27843 WP_014727406.1 NC_017927:668405-668770 [Streptococcus thermophilus MN-ZLW-002]
VHAIYFYKDKQGNQPVLDYMRELARHDSKDSRIKLNKLNDYIELLSQHGTCAGQPYIKHLDAEIWELRPLRDRILFVAWL
DGSFVLLHHFVKKTQKTPRREIEKAKRELQDLKERGLRDEE

Nucleotide

• Length: 366 bp

>T27843 NC_017927:668405-668770 [Streptococcus thermophilus MN-ZLW-002]
GTGCACGCTATTTATTTTTATAAGGACAAACAGGGGAATCAACCGGTCTTGGACTACATGAGAGAGCTAGCTCGTCACGA
TAGCAAGGACAGCCGGATCAAACTCAACAAGCTCAACGACTACATCGAACTTTTGAGCCAACATGGTACCTGTGCTGGCC
AACCCTATATCAAGCATCTGGATGCGGAGATTTGGGAGTTGAGACCCTTGCGGGATCGGATTTTATTCGTGGCTTGGTTG
GATGGAAGCTTTGTCTTACTGCATCATTTTGTCAAAAAGACGCAAAAGACACCTAGACGAGAGATCGAGAAAGCCAAGCG
GGAGTTGCAAGATTTGAAGGAAAGAGGGCTAAGAGATGAAGAATAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10778.52 Da
• Isoelectric Point: 5.8962

>AT27843 WP_002890617.1 NC_017927:668760-669053 [Streptococcus thermophilus MN-ZLW-002]
MKNSAIGSNWKDVRAELFTKEEILESDLRVAIMTELIEARHEKGFSQKKLEELSGVSQPVIARMETGKTSPQLDTVLKVL
ASLGKTLAVVPLEQEKL

Nucleotide

• Length: 294 bp

>AT27843 NC_017927:668760-669053 [Streptococcus thermophilus MN-ZLW-002]
ATGAAGAATAGTGCCATCGGCAGCAATTGGAAAGATGTGAGAGCAGAGCTCTTCACAAAGGAAGAAATCCTGGAAAGTGA
TCTGCGGGTCGCGATTATGACGGAGCTAATCGAGGCTCGGCATGAAAAGGGCTTTAGCCAAAAGAAACTAGAAGAACTCA
GTGGGGTCAGTCAACCGGTCATCGCTCGGATGGAGACGGGAAAGACCAGTCCTCAATTGGACACGGTCTTGAAAGTCTTA
GCCAGTCTGGGCAAGACCCTTGCTGTCGTCCCACTGGAACAAGAAAAGCTCTAA

Structures

Antitoxin

• AlphaFold DB: J7TXL7