Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1111669..1112396 Replicon chromosome
Accession NZ_CP123013
Organism Escherichia coli strain 18-4

Toxin (Protein)


Gene name higB Uniprot ID J7Q991
Locus tag QEN56_RS05445 Protein ID WP_000547564.1
Coordinates 1111669..1111980 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag QEN56_RS05450 Protein ID WP_000126294.1
Coordinates 1111977..1112396 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QEN56_RS05415 (1106811) 1106811..1108520 + 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -
QEN56_RS05420 (1108530) 1108530..1109072 + 543 WP_000493792.1 formate hydrogenlyase subunit HycF -
QEN56_RS05425 (1109072) 1109072..1109839 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
QEN56_RS05430 (1109836) 1109836..1110246 + 411 WP_001291921.1 formate hydrogenlyase assembly protein HycH -
QEN56_RS05435 (1110239) 1110239..1110709 + 471 WP_060621146.1 hydrogenase maturation peptidase HycI -
QEN56_RS05440 (1110734) 1110734..1111495 + 762 WP_001026446.1 hypothetical protein -
QEN56_RS05445 (1111669) 1111669..1111980 + 312 WP_000547564.1 type II toxin-antitoxin system HigB family toxin Toxin
QEN56_RS05450 (1111977) 1111977..1112396 + 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
QEN56_RS05455 (1112510) 1112510..1113934 - 1425 WP_000110363.1 6-phospho-beta-glucosidase AscB -
QEN56_RS05460 (1113943) 1113943..1115400 - 1458 WP_001107861.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
QEN56_RS05465 (1115660) 1115660..1116670 + 1011 WP_001392554.1 DNA-binding transcriptional regulator AscG -
QEN56_RS05470 (1116819) 1116819..1117346 + 528 WP_001078777.1 electron transport protein HydN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12426.13 Da        Isoelectric Point: 9.7248

>T278414 WP_000547564.1 NZ_CP123013:1111669-1111980 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT278414 WP_000126294.1 NZ_CP123013:1111977-1112396 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FJG5

Antitoxin

Source ID Structure

References