Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4546819..4547618 Replicon chromosome
Accession NZ_CP123008
Organism Escherichia coli strain NWU_4

Toxin (Protein)


Gene name yhaV Uniprot ID F4VJD3
Locus tag QEN27_RS21920 Protein ID WP_000347266.1
Coordinates 4546819..4547283 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag QEN27_RS21925 Protein ID WP_001307405.1
Coordinates 4547283..4547618 (-) Length 112 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QEN27_RS21890 (4541820) 4541820..4542254 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
QEN27_RS21895 (4542272) 4542272..4543150 - 879 WP_001315856.1 PTS N-acetylgalactosamine transporter subunit IID -
QEN27_RS21900 (4543140) 4543140..4543919 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
QEN27_RS21905 (4543930) 4543930..4544403 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
QEN27_RS21910 (4544426) 4544426..4545706 - 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
QEN27_RS21915 (4545955) 4545955..4546764 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
QEN27_RS21920 (4546819) 4546819..4547283 - 465 WP_000347266.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
QEN27_RS21925 (4547283) 4547283..4547618 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
QEN27_RS21930 (4547767) 4547767..4549338 - 1572 WP_001273741.1 galactarate dehydratase -
QEN27_RS21935 (4549713) 4549713..4551047 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
QEN27_RS21940 (4551063) 4551063..4551833 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(12-150)

Antitoxin

(14-110)


Sequences


Toxin        


Download         Length: 155 a.a.        Molecular weight: 17841.18 Da        Isoelectric Point: 9.4947

>T278380 WP_000347266.1 NZ_CP123008:c4547283-4546819 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T278380 NZ_CP123008:c4547283-4546819 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTTTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT278380 WP_001307405.1 NZ_CP123008:c4547618-4547283 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT278380 NZ_CP123008:c4547618-4547283 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A836NGD2


Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References