Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2066..2712 Replicon plasmid unnamed5
Accession NZ_CP122934
Organism Escherichia coli strain ETEC1702

Toxin (Protein)


Gene name higB Uniprot ID F4T8Q4
Locus tag QDX05_RS25045 Protein ID WP_000269926.1
Coordinates 2066..2416 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID F4T8Q5
Locus tag QDX05_RS25050 Protein ID WP_001259443.1
Coordinates 2413..2712 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QDX05_RS25025 (QDX05_25020) 1..306 + 306 Protein_0 hypothetical protein -
QDX05_RS25030 (QDX05_25025) 485..748 + 264 WP_001312455.1 PilI type IV pilus biogenesis protein -
QDX05_RS25035 (QDX05_25030) 780..1013 - 234 WP_000024515.1 hypothetical protein -
QDX05_RS25040 (QDX05_25035) 1053..1700 - 648 WP_000214917.1 ParA family protein -
QDX05_RS25045 (QDX05_25040) 2066..2416 + 351 WP_000269926.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
QDX05_RS25050 (QDX05_25045) 2413..2712 + 300 WP_001259443.1 XRE family transcriptional regulator Antitoxin
QDX05_RS25055 (QDX05_25050) 2743..2988 - 246 WP_001193619.1 hypothetical protein -
QDX05_RS25060 (QDX05_25055) 2978..3235 - 258 WP_000845367.1 hypothetical protein -
QDX05_RS25065 (QDX05_25060) 3271..3813 - 543 WP_000206647.1 hypothetical protein -
QDX05_RS25070 (QDX05_25065) 3941..4474 - 534 WP_001025389.1 J domain-containing protein -
QDX05_RS25075 (QDX05_25070) 4845..5144 - 300 WP_000113288.1 hypothetical protein -
QDX05_RS25080 (QDX05_25075) 5218..5490 - 273 WP_001272119.1 hypothetical protein -
QDX05_RS25085 (QDX05_25080) 6441..7106 - 666 WP_001350943.1 RepA family replication protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..24405 24405


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-105)

Antitoxin

Cro/C1-type helix-turn-helix domain

(32-80)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13742.77 Da        Isoelectric Point: 7.4217

>T278256 WP_000269926.1 NZ_CP122934:2066-2416 [Escherichia coli]
MWTVYFGRLFDEWFEEQELALKRKVLAELKHLEEFGPSLSRPHADTVKGSRHKNMKELRIQYEGHPIRAFFAFDPIRQAI
ILCAGDKSNDKKFYDRMIRIADEEFSTHLAELEGKK

Download         Length: 351 bp

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10917.70 Da        Isoelectric Point: 4.6283

>AT278256 WP_001259443.1 NZ_CP122934:2413-2712 [Escherichia coli]
MRTLEEVIAAQSPESQAIIKAMADEMILETGLQMMREELQLSQKQVADAMGISQPAVTQLEQRGNDLKLATLKRYVEAMG
GKLSLDVELPTGKRVAFNI

Download         Length: 300 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A743U745

Antitoxin

Source ID Structure
AlphaFold DB A0A743U677

References