Detailed information of TA system TA277989

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-agrB/-
• Location: 32864..33289
• Replicon: plasmid unnamed7
• Accession: NZ_CP122830
• Organism: Escherichia coli strain ETEC1732

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: QDX01_RS28300
• Protein ID: WP_223195199.1
• Coordinates: 33167..33289 (+)
• Length: 41 a.a.

Antitoxin (RNA)

Gene name	agrB
Locus tag	-
Coordinates	32864..33075 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QDX01_RS28265 (28574)	28574..28780	+	207	WP_000547945.1	hypothetical protein	-
QDX01_RS28270 (28806)	28806..29336	+	531	WP_032214168.1	single-stranded DNA-binding protein	-
QDX01_RS28275 (29392)	29392..29625	+	234	WP_000005971.1	DUF905 domain-containing protein	-
QDX01_RS28280 (29689)	29689..31647	+	1959	WP_282835507.1	ParB/RepB/Spo0J family partition protein	-
QDX01_RS28285 (31702)	31702..32136	+	435	WP_042062942.1	conjugation system SOS inhibitor PsiB	-
QDX01_RS28290 (32133)	32133..32919	+	787	Protein_44	plasmid SOS inhibition protein A	-
- (32864)	32864..33075	+	212	NuclAT_0	-	Antitoxin
- (32864)	32864..33075	+	212	NuclAT_0	-	Antitoxin
- (32864)	32864..33075	+	212	NuclAT_0	-	Antitoxin
- (32864)	32864..33075	+	212	NuclAT_0	-	Antitoxin
QDX01_RS28295 (33109)	33109..33222	+	114	Protein_45	DUF5431 family protein	-
QDX01_RS28300 (33167)	33167..33289	+	123	WP_223195199.1	Hok/Gef family protein	Toxin
QDX01_RS28305 (33645)	33645..35216	-	1572	WP_000381443.1	IS66 family transposase	-
QDX01_RS28310 (35236)	35236..35583	-	348	WP_000624618.1	IS66 family insertion sequence element accessory protein TnpB	-
QDX01_RS28315 (35583)	35583..36233	-	651	WP_000993956.1	IS66-like element accessory protein TnpA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	estIa / hlyD / hlyB / hlyB / hlyA / hlyC	1..98230	98230

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-35)

Sequences

Toxin

Protein

• Length: 41 a.a.
• Molecular weight: 4536.35 Da
• Isoelectric Point: 8.2691

>T277989 WP_223195199.1 NZ_CP122830:33167-33289 [Escherichia coli]
IVCCTLLIFTLLTRNRLCEVRLKDGYREVTASLAYESSGK

Nucleotide

• Length: 123 bp

Antitoxin

Nucleotide

• Length: 212 bp

>AT277989 NZ_CP122830:32864-33075 [Escherichia coli]
TCACACGGATTACCCGTGAACTGGTTGAACGACCAGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
AGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGC
AGAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGCCCCCTGTATGTCT

Structures

Antitoxin