Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1151256..1151983 Replicon chromosome
Accession NZ_CP122816
Organism Escherichia coli strain ETEC1731

Toxin (Protein)


Gene name higB Uniprot ID J7Q991
Locus tag QDY29_RS05680 Protein ID WP_000547564.1
Coordinates 1151256..1151567 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag QDY29_RS05685 Protein ID WP_000126294.1
Coordinates 1151564..1151983 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QDY29_RS05650 (1146398) 1146398..1148107 + 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -
QDY29_RS05655 (1148117) 1148117..1148659 + 543 WP_000493792.1 formate hydrogenlyase subunit HycF -
QDY29_RS05660 (1148659) 1148659..1149426 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
QDY29_RS05665 (1149423) 1149423..1149833 + 411 WP_001291921.1 formate hydrogenlyase assembly protein HycH -
QDY29_RS05670 (1149826) 1149826..1150296 + 471 WP_000132961.1 hydrogenase maturation peptidase HycI -
QDY29_RS05675 (1150321) 1150321..1151082 + 762 WP_001026446.1 hypothetical protein -
QDY29_RS05680 (1151256) 1151256..1151567 + 312 WP_000547564.1 type II toxin-antitoxin system HigB family toxin Toxin
QDY29_RS05685 (1151564) 1151564..1151983 + 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
QDY29_RS05690 (1152097) 1152097..1153521 - 1425 WP_000110313.1 6-phospho-beta-glucosidase AscB -
QDY29_RS05695 (1153530) 1153530..1154987 - 1458 WP_001107856.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
QDY29_RS05700 (1155247) 1155247..1156257 + 1011 WP_001363554.1 DNA-binding transcriptional regulator AscG -
QDY29_RS05705 (1156406) 1156406..1156933 + 528 WP_001078777.1 electron transport protein HydN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12426.13 Da        Isoelectric Point: 9.7248

>T277933 WP_000547564.1 NZ_CP122816:1151256-1151567 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT277933 WP_000126294.1 NZ_CP122816:1151564-1151983 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FJG5

Antitoxin

Source ID Structure

References