Detailed information of TA system TA277920

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-agrB/-
• Location: 32862..33287
• Replicon: plasmid unnamed6
• Accession: NZ_CP122807
• Organism: Escherichia coli strain ETEC1734

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: QDX84_RS27705
• Protein ID: WP_223195199.1
• Coordinates: 33165..33287 (+)
• Length: 41 a.a.

Antitoxin (RNA)

Gene name	agrB
Locus tag	-
Coordinates	32862..33073 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QDX84_RS27670 (28572)	28572..28778	+	207	WP_000547945.1	hypothetical protein	-
QDX84_RS27675 (28804)	28804..29334	+	531	WP_032214168.1	single-stranded DNA-binding protein	-
QDX84_RS27680 (29390)	29390..29623	+	234	WP_000005971.1	DUF905 domain-containing protein	-
QDX84_RS27685 (29687)	29687..31645	+	1959	WP_282835507.1	ParB/RepB/Spo0J family partition protein	-
QDX84_RS27690 (31700)	31700..32134	+	435	WP_042062942.1	conjugation system SOS inhibitor PsiB	-
QDX84_RS27695 (32131)	32131..32917	+	787	Protein_43	plasmid SOS inhibition protein A	-
- (32862)	32862..33073	+	212	NuclAT_0	-	Antitoxin
- (32862)	32862..33073	+	212	NuclAT_0	-	Antitoxin
- (32862)	32862..33073	+	212	NuclAT_0	-	Antitoxin
- (32862)	32862..33073	+	212	NuclAT_0	-	Antitoxin
QDX84_RS27700 (33107)	33107..33220	+	114	Protein_44	DUF5431 family protein	-
QDX84_RS27705 (33165)	33165..33287	+	123	WP_223195199.1	Hok/Gef family protein	Toxin
QDX84_RS27710 (33643)	33643..35214	-	1572	WP_000381443.1	IS66 family transposase	-
QDX84_RS27715 (35234)	35234..35581	-	348	WP_000624618.1	IS66 family insertion sequence element accessory protein TnpB	-
QDX84_RS27720 (35581)	35581..36231	-	651	WP_000993956.1	IS66-like element accessory protein TnpA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	estIa / hlyD / hlyB / hlyB / hlyA / hlyC	1..98226	98226

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-35)

Sequences

Toxin

Protein

• Length: 41 a.a.
• Molecular weight: 4536.35 Da
• Isoelectric Point: 8.2691

>T277920 WP_223195199.1 NZ_CP122807:33165-33287 [Escherichia coli]
IVCCTLLIFTLLTRNRLCEVRLKDGYREVTASLAYESSGK

Nucleotide

• Length: 123 bp

Antitoxin

Nucleotide

• Length: 212 bp

>AT277920 NZ_CP122807:32862-33073 [Escherichia coli]
TCACACGGATTACCCGTGAACTGGTTGAACGACCAGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
AGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGC
AGAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGCCCCCTGTATGTCT

Structures

Antitoxin