Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1214337..1215064 Replicon chromosome
Accession NZ_CP122768
Organism Escherichia coli strain ETEC1738

Toxin (Protein)


Gene name higB Uniprot ID D6JE58
Locus tag QDY55_RS05890 Protein ID WP_000547557.1
Coordinates 1214337..1214648 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1M2G9D6
Locus tag QDY55_RS05895 Protein ID WP_001600394.1
Coordinates 1214645..1215064 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QDY55_RS05860 (1209479) 1209479..1211188 + 1710 WP_064225926.1 formate hydrogenlyase subunit HycE -
QDY55_RS05865 (1211198) 1211198..1211740 + 543 WP_000493779.1 formate hydrogenlyase subunit HycF -
QDY55_RS05870 (1211740) 1211740..1212507 + 768 WP_000067401.1 formate hydrogenlyase subunit HycG -
QDY55_RS05875 (1212504) 1212504..1212914 + 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
QDY55_RS05880 (1212907) 1212907..1213377 + 471 WP_000132947.1 hydrogenase maturation peptidase HycI -
QDY55_RS05885 (1213420) 1213420..1214175 + 756 WP_064225927.1 hypothetical protein -
QDY55_RS05890 (1214337) 1214337..1214648 + 312 WP_000547557.1 type II toxin-antitoxin system HigB family toxin Toxin
QDY55_RS05895 (1214645) 1214645..1215064 + 420 WP_001600394.1 helix-turn-helix domain-containing protein Antitoxin
QDY55_RS05900 (1215143) 1215143..1216567 - 1425 WP_064225940.1 6-phospho-beta-glucosidase AscB -
QDY55_RS05905 (1216576) 1216576..1218033 - 1458 WP_064225928.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
QDY55_RS05910 (1218293) 1218293..1219303 + 1011 WP_024187449.1 DNA-binding transcriptional regulator AscG -
QDY55_RS05915 (1219452) 1219452..1219979 + 528 WP_001078777.1 electron transport protein HydN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12467.20 Da        Isoelectric Point: 9.5262

>T277865 WP_000547557.1 NZ_CP122768:1214337-1214648 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15389.40 Da        Isoelectric Point: 4.4596

>AT277865 WP_001600394.1 NZ_CP122768:1214645-1215064 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYESNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB D6JE58

Antitoxin

Source ID Structure
AlphaFold DB A0A1M2G9D6

References