Detailed information of TA system TA027764

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 289897..290591
• Replicon: chromosome
• Accession: NC_017906
• Organism: Escherichia coli Xuzhou21

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: CDCO157_RS01355
• Protein ID: WP_001263495.1
• Coordinates: 290193..290591 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: CDCO157_RS01350
• Protein ID: WP_000554757.1
• Coordinates: 289897..290190 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDCO157_RS31785	285608..286105	+	498	Protein_249	REP-associated tyrosine transposase RayT	-
CDCO157_RS01335	286250..287962	-	1713	Protein_250	flagellar biosynthesis protein FlhA	-
CDCO157_RS01340	287934..288719	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
CDCO157_RS01345	288790..289845	+	1056	WP_001226188.1	DNA polymerase IV	-
CDCO157_RS01350	289897..290190	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
CDCO157_RS01355	290193..290591	+	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
CDCO157_RS01360	290601..291053	+	453	WP_001059871.1	GNAT family N-acetyltransferase	-
CDCO157_RS01365	291372..291575	+	204	Protein_256	RNA ligase RtcB family protein	-
CDCO157_RS01370	291574..292095	+	522	Protein_257	peptide chain release factor H	-
CDCO157_RS01375	292152..293609	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
CDCO157_RS01380	293870..294328	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
-	294924..295004	+	81	NuclAT_10	-	-
-	294924..295004	+	81	NuclAT_10	-	-
-	294924..295004	+	81	NuclAT_10	-	-
-	294924..295004	+	81	NuclAT_10	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T27764 WP_001263495.1 NC_017906:290193-290591 [Escherichia coli Xuzhou21]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T27764 NC_017906:290193-290591 [Escherichia coli Xuzhou21]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT27764 WP_000554757.1 NC_017906:289897-290190 [Escherichia coli Xuzhou21]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT27764 NC_017906:289897-290190 [Escherichia coli Xuzhou21]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6