Detailed information of TA system TA027763

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 283934..284473
• Replicon: chromosome
• Accession: NC_017906
• Organism: Escherichia coli Xuzhou21

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: -
• Locus tag: CDCO157_RS01315
• Protein ID: WP_001303998.1
• Coordinates: 283934..284194 (-)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: C3TP07
• Locus tag: CDCO157_RS01320
• Protein ID: WP_000729705.1
• Coordinates: 284213..284473 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDCO157_RS01300	281617..282195	+	579	WP_000284050.1	D-sedoheptulose 7-phosphate isomerase	-
CDCO157_RS01305	282300..283067	+	768	WP_001301698.1	class II glutamine amidotransferase	-
CDCO157_RS01310	283038..283778	-	741	WP_001225679.1	murein L,D-transpeptidase	-
CDCO157_RS01315	283934..284194	-	261	WP_001303998.1	type II toxin-antitoxin system mRNA interferase toxin YafQ	Toxin
CDCO157_RS01320	284213..284473	-	261	WP_000729705.1	type II toxin-antitoxin system antitoxin DinJ	Antitoxin
CDCO157_RS01325	284659..285432	+	774	WP_000543891.1	C40 family peptidase	-
CDCO157_RS31785	285608..286105	+	498	Protein_249	REP-associated tyrosine transposase RayT	-
CDCO157_RS01335	286250..287962	-	1713	Protein_250	flagellar biosynthesis protein FlhA	-
CDCO157_RS01340	287934..288719	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-85)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-85)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10026.71 Da
• Isoelectric Point: 10.3188

>T27763 WP_001303998.1 NC_017906:c284194-283934 [Escherichia coli Xuzhou21]
MNSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGT
HAALFG

Nucleotide

• Length: 261 bp

>T27763 NC_017906:c284194-283934 [Escherichia coli Xuzhou21]
TTGAACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAATAAATTGAAATATCTTAT
GACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAGGTTCATGGAAAGGTTATC
GCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGATTTGAGAGAACTGGAACT
CACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9377.74 Da
• Isoelectric Point: 5.0758

>AT27763 WP_000729705.1 NC_017906:c284473-284213 [Escherichia coli Xuzhou21]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGV

Nucleotide

• Length: 261 bp

>AT27763 NC_017906:c284473-284213 [Escherichia coli Xuzhou21]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAGTTTAA