Detailed information of TA system TA027760

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 577154..577666
• Replicon: chromosome
• Accession: NC_017905
• Organism: Streptococcus parasanguinis FW213

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: I1ZKJ4
• Locus tag: SPAF_RS03010
• Protein ID: WP_013903192.1
• Coordinates: 577412..577666 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: SPAF_RS03005
• Protein ID: WP_003014291.1
• Coordinates: 577154..577408 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SPAF_RS02970	572330..572641	+	312	WP_003005853.1	ribosome assembly RNA-binding protein YhbY	-
SPAF_RS02975	572663..573295	+	633	WP_003012335.1	nicotinate-nucleotide adenylyltransferase	-
SPAF_RS02980	573292..573888	+	597	WP_013903187.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
SPAF_RS02985	573889..574389	+	501	WP_013903188.1	cysteine hydrolase	-
SPAF_RS02990	574404..574757	+	354	WP_003014310.1	ribosome silencing factor	-
SPAF_RS02995	575011..575820	+	810	WP_013903189.1	class I SAM-dependent methyltransferase	-
SPAF_RS03000	575827..576921	+	1095	WP_013903190.1	nucleotidyltransferase	-
SPAF_RS03005	577154..577408	+	255	WP_003014291.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
SPAF_RS03010	577412..577666	+	255	WP_013903192.1	Txe/YoeB family addiction module toxin	Toxin
SPAF_RS03015	577841..578557	+	717	WP_003005873.1	YebC/PmpR family DNA-binding transcriptional regulator	-
SPAF_RS03020	578610..578936	-	327	WP_009732369.1	hypothetical protein	-
SPAF_RS03025	578917..579288	-	372	WP_013903193.1	hypothetical protein	-
SPAF_RS03030	579285..580697	-	1413	WP_014712964.1	Y-family DNA polymerase	-
SPAF_RS03035	580700..581377	-	678	WP_041826243.1	XRE family transcriptional regulator	-
SPAF_RS03040	581537..582529	+	993	WP_003005888.1	aspartate--ammonia ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (1-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-75)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10082.55 Da
• Isoelectric Point: 7.9789

>T27760 WP_013903192.1 NC_017905:577412-577666 [Streptococcus parasanguinis FW213]
MLLKFTEDAWKDYCYWQSQDKKTLKRINTLIKEIQRDPFAGIGKPEPLKYDFQGAWSRRIDAENRLIYMLDESGVAFLSF
KDHY

Nucleotide

• Length: 255 bp

>T27760 NC_017905:577412-577666 [Streptococcus parasanguinis FW213]
ATGTTACTCAAGTTTACAGAAGATGCCTGGAAGGATTATTGTTACTGGCAAAGTCAGGACAAAAAAACACTGAAGCGAAT
CAATACCCTTATCAAAGAGATCCAAAGGGACCCATTTGCAGGGATTGGTAAGCCTGAGCCTTTGAAATATGACTTTCAAG
GAGCATGGTCAAGACGGATTGATGCAGAGAATCGCCTGATTTATATGCTAGATGAGTCAGGTGTCGCTTTCCTCTCTTTT
AAAGATCATTATTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9703.98 Da
• Isoelectric Point: 4.6768

>AT27760 WP_003014291.1 NC_017905:577154-577408 [Streptococcus parasanguinis FW213]
MEAVVYSTFRNHLKDYMKKVNDEFEPLTVVNKNPDEDIVVISKSEWDSLQETLRLAQNKELSDKVLRGMAEVRAGQVQLH
EIEG

Nucleotide

• Length: 255 bp

>AT27760 NC_017905:577154-577408 [Streptococcus parasanguinis FW213]
ATGGAAGCAGTCGTTTATTCAACATTCCGCAATCATTTGAAGGATTATATGAAGAAGGTGAATGATGAGTTTGAGCCTTT
GACAGTCGTGAATAAGAATCCAGACGAAGATATTGTGGTCATCTCTAAGAGCGAGTGGGACAGCCTACAAGAAACCCTGA
GACTAGCTCAGAACAAGGAATTGTCAGATAAGGTCTTGCGGGGCATGGCAGAAGTCCGAGCTGGTCAGGTTCAACTCCAT
GAGATTGAGGGGTAA

Structures

Toxin

• AlphaFold DB: I1ZKJ4