Detailed information of TA system TA277563

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2725454..2725825
• Replicon: chromosome
• Accession: NZ_CP122655
• Organism: Escherichia coli strain ETEC4074

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: -
• Locus tag: QDW60_RS13725
• Protein ID: WP_032083637.1
• Coordinates: 2725454..2725648 (+)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2725647..2725825 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QDW60_RS13705 (2720556)	2720556..2720732	+	177	WP_000241145.1	YdaE family protein	-
QDW60_RS13710 (2720806)	2720806..2721081	+	276	WP_000632298.1	hypothetical protein	-
QDW60_RS13715 (2721183)	2721183..2724326	+	3144	WP_032083635.1	exodeoxyribonuclease VIII	-
QDW60_RS13720 (2724338)	2724338..2725390	+	1053	WP_032083636.1	RecT family recombinase	-
QDW60_RS13725 (2725454)	2725454..2725648	+	195	WP_032083637.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
- (2725647)	2725647..2725825	-	179	NuclAT_8	-	Antitoxin
- (2725647)	2725647..2725825	-	179	NuclAT_8	-	Antitoxin
- (2725647)	2725647..2725825	-	179	NuclAT_8	-	Antitoxin
- (2725647)	2725647..2725825	-	179	NuclAT_8	-	Antitoxin
QDW60_RS13730 (2725641)	2725641..2725829	+	189	WP_001602382.1	DUF1187 family protein	-
QDW60_RS13735 (2725929)	2725929..2726144	+	216	WP_000079604.1	excisionase XisR	-
QDW60_RS13740 (2726146)	2726146..2727381	+	1236	WP_072014650.1	site-specific integrase	-
QDW60_RS13745 (2727433)	2727433..2728368	+	936	WP_001157377.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
QDW60_RS13750 (2728497)	2728497..2729870	-	1374	WP_000123737.1	ATP-dependent RNA helicase DbpA	-
QDW60_RS13755 (2729900)	2729900..2730073	-	174	WP_001296046.1	protein YnaL	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2678586..2731331	52745
	-	inside	Prophage	-	-	2678586..2734640	56054

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7107.02 Da
• Isoelectric Point: 9.5240

>T277563 WP_032083637.1 NZ_CP122655:2725454-2725648 [Escherichia coli]
MRYEKVKPYPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

Antitoxin

Nucleotide

• Length: 179 bp

>AT277563 NZ_CP122655:c2725825-2725647 [Escherichia coli]
GAAGATTGAAGTTTCTCGCAATTAAAATTTATAAGTTTTACTTTCTGCTCTCTGGAAACACCAGCTTCTTTTTTCCCTGA
GAGCATTTTTTCGCATTCCGATTTGGTTAACTTTGTTGTTGAGTACCTTGTCCAGCTGGTAGGAGAACCTCCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Antitoxin