Detailed information of TA system TA027756

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Gp49-HTH_37
• Location: 1873253..1874233
• Replicon: chromosome
• Accession: NC_017904
• Organism: Mycobacterium sp. MOTT36Y

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I2ABQ1
• Locus tag: W7S_RS08760
• Protein ID: WP_014711308.1
• Coordinates: 1873850..1874233 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: W7S_RS25700
• Protein ID: WP_014711307.1
• Coordinates: 1873253..1873744 (-)
• Length: 164 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
W7S_RS08735	1868462..1869568	-	1107	WP_041298200.1	site-specific integrase	-
W7S_RS08740	1870021..1871574	+	1554	WP_041297812.1	IS21 family transposase	-
W7S_RS08745	1871571..1872383	+	813	WP_014711299.1	ATP-binding protein	-
W7S_RS08750	1872744..1873256	-	513	WP_014711306.1	SecB chaperone Rv1957	-
W7S_RS25700	1873253..1873744	-	492	WP_014711307.1	helix-turn-helix domain-containing protein	Antitoxin
W7S_RS08760	1873850..1874233	-	384	WP_014711308.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
W7S_RS08765	1874732..1875001	+	270	WP_014711309.1	hypothetical protein	-
W7S_RS08770	1875032..1878127	+	3096	WP_014711310.1	DEAD/DEAH box helicase	-
W7S_RS08775	1878161..1879198	+	1038	WP_148277768.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1823379..1879198	55819

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (29-115)

Antitoxin

• Cro/C1-type helix-turn-helix domain (52-94)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14770.00 Da
• Isoelectric Point: 11.5672

>T27756 WP_014711308.1 NC_017904:c1874233-1873850 [Mycobacterium sp. MOTT36Y]
VSTQHSGPAREGKWQFFRASTKGRPIFEKELDKLPRDARAALANLMRRYLLGQLRPGDLKPVQGGISELRWRDGNDHYRV
LFFRWGCHPVALVAFFKNQQRTPKSKVDLAKKRRKAWTDAFRDHPAT

Nucleotide

• Length: 384 bp

>T27756 NC_017904:c1874233-1873850 [Mycobacterium sp. MOTT36Y]
GTGTCGACGCAACACAGCGGACCAGCTCGCGAGGGCAAGTGGCAGTTCTTTCGCGCATCCACCAAGGGACGGCCAATTTT
TGAGAAGGAGCTGGACAAGCTTCCTCGCGATGCGCGGGCAGCGCTCGCTAACCTCATGCGGCGATATTTGTTAGGGCAAC
TGCGGCCCGGGGATCTCAAACCTGTTCAAGGTGGAATTAGCGAGCTTCGTTGGCGCGACGGCAACGACCACTACAGGGTG
TTGTTCTTCCGGTGGGGTTGCCACCCAGTTGCTCTAGTGGCGTTTTTCAAGAACCAGCAACGAACCCCCAAGTCCAAGGT
TGACCTTGCCAAGAAGCGCCGCAAGGCGTGGACCGATGCCTTCAGAGATCACCCCGCCACCTAG

Antitoxin

Protein

• Length: 164 a.a.
• Molecular weight: 18203.23 Da
• Isoelectric Point: 5.2961

>AT27756 WP_014711307.1 NC_017904:c1873744-1873253 [Mycobacterium sp. MOTT36Y]
MITGSQGTETSVPLTDDLERYIEETSDADPGFKVALEDAEELQRLLDTLITLRRERHLSQTDVAERMGVRQPTVSGFEKQ
SDPRMSTLQRYARAVEARLRFVVENPSTCGWVSPGTQAYLRGQTTQVGSSTHKNCKILATWRHTSDESSGTWASSRLDWV
GVA

Nucleotide

• Length: 492 bp

>AT27756 NC_017904:c1873744-1873253 [Mycobacterium sp. MOTT36Y]
ATGATCACCGGAAGCCAAGGCACCGAAACGTCTGTGCCGTTGACCGACGATCTTGAGCGCTACATCGAGGAGACCTCGGA
CGCCGACCCTGGTTTTAAGGTTGCGCTTGAGGACGCCGAGGAACTCCAGCGGCTCTTGGACACGCTGATCACGCTCCGCA
GAGAACGACACCTCAGCCAGACCGACGTTGCTGAGCGCATGGGTGTACGTCAACCGACGGTGAGTGGATTTGAGAAGCAG
AGCGACCCGCGTATGTCCACGCTTCAGCGTTACGCTCGCGCGGTCGAAGCTCGATTGCGCTTTGTTGTGGAGAACCCCTC
GACGTGTGGCTGGGTCTCGCCAGGAACGCAAGCTTATCTTCGAGGTCAAACGACACAGGTAGGTTCGTCGACACATAAGA
ACTGCAAAATTCTTGCAACCTGGAGGCACACGTCGGACGAGTCGTCGGGTACCTGGGCCAGCAGCCGTTTGGACTGGGTC
GGAGTGGCGTGA

Structures

Toxin

• AlphaFold DB: I2ABQ1