277456

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4201355..4202049 Replicon chromosome
Accession NZ_CP122634
Organism Escherichia coli strain ETEC4085

Toxin (Protein)


Gene name yafO Uniprot ID A0A3A6T3I6
Locus tag QDW62_RS21210 Protein ID WP_001263490.1
Coordinates 4201355..4201753 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1FJN6
Locus tag QDW62_RS21215 Protein ID WP_000554757.1
Coordinates 4201756..4202049 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (4197015) 4197015..4197095 - 81 NuclAT_9 - -
- (4197015) 4197015..4197095 - 81 NuclAT_9 - -
- (4197015) 4197015..4197095 - 81 NuclAT_9 - -
- (4197015) 4197015..4197095 - 81 NuclAT_9 - -
QDW62_RS21180 (4196355) 4196355..4197599 - 1245 WP_000189541.1 esterase FrsA -
QDW62_RS21185 (4197691) 4197691..4198149 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
QDW62_RS21190 (4198410) 4198410..4199867 + 1458 WP_001293003.1 cytosol nonspecific dipeptidase -
QDW62_RS21195 (4199924) 4199924..4200445 - 522 Protein_4148 peptide chain release factor H -
QDW62_RS21200 (4200444) 4200444..4200647 - 204 Protein_4149 RtcB family protein -
QDW62_RS21205 (4200893) 4200893..4201345 - 453 WP_001059847.1 GNAT family N-acetyltransferase -
QDW62_RS21210 (4201355) 4201355..4201753 - 399 WP_001263490.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
QDW62_RS21215 (4201756) 4201756..4202049 - 294 WP_000554757.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
QDW62_RS21220 (4202101) 4202101..4203156 - 1056 WP_001226164.1 DNA polymerase IV -
QDW62_RS21225 (4203227) 4203227..4204012 - 786 WP_000207587.1 putative lateral flagellar export/assembly protein LafU -
QDW62_RS21230 (4203984) 4203984..4205696 + 1713 Protein_4155 flagellar biosynthesis protein FlhA -
QDW62_RS21235 (4205920) 4205920..4206417 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 4153747..4207219 53472


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15486.86 Da        Isoelectric Point: 8.0949

>T277456 WP_001263490.1 NZ_CP122634:c4201753-4201355 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLLYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.81 Da        Isoelectric Point: 5.9050

>AT277456 WP_000554757.1 NZ_CP122634:c4202049-4201756 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3A6T3I6

Antitoxin

Source ID Structure
AlphaFold DB S1FJN6

References