Detailed information of TA system TA277433

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 105557..105982
• Replicon: plasmid unnamed4
• Accession: NZ_CP122633
• Organism: Escherichia coli strain ETEC4088

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: QDW58_RS25345
• Protein ID: WP_223195199.1
• Coordinates: 105860..105982 (+)
• Length: 41 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	105557..105768 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QDW58_RS25310 (100770)	100770..101189	-	420	WP_015056142.1	hypothetical protein	-
QDW58_RS25315 (101490)	101490..101987	+	498	Protein_95	single-stranded DNA-binding protein	-
QDW58_RS25320 (102085)	102085..102318	+	234	WP_000005988.1	DUF905 family protein	-
QDW58_RS25325 (102382)	102382..104340	+	1959	WP_282834335.1	ParB/RepB/Spo0J family partition protein	-
QDW58_RS25330 (104395)	104395..104829	+	435	WP_000845922.1	conjugation system SOS inhibitor PsiB	-
QDW58_RS25335 (104826)	104826..105612	+	787	Protein_99	plasmid SOS inhibition protein A	-
- (105557)	105557..105768	+	212	NuclAT_0	-	Antitoxin
- (105557)	105557..105768	+	212	NuclAT_0	-	Antitoxin
- (105557)	105557..105768	+	212	NuclAT_0	-	Antitoxin
- (105557)	105557..105768	+	212	NuclAT_0	-	Antitoxin
QDW58_RS25340 (105802)	105802..105915	+	114	Protein_100	DUF5431 family protein	-
QDW58_RS25345 (105860)	105860..105982	+	123	WP_223195199.1	Hok/Gef family protein	Toxin
QDW58_RS25350 (106283)	106283..106580	-	298	Protein_102	hypothetical protein	-
QDW58_RS25355 (106881)	106881..107168	+	288	WP_000107520.1	hypothetical protein	-
QDW58_RS25360 (107287)	107287..108108	+	822	WP_001234468.1	DUF932 domain-containing protein	-
QDW58_RS25365 (108404)	108404..108913	-	510	WP_249820740.1	transglycosylase SLT domain-containing protein	-
QDW58_RS25370 (109329)	109329..109712	+	384	WP_098723228.1	conjugal transfer relaxosome DNA-binding protein TraM	-
QDW58_RS25375 (109899)	109899..110588	+	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	vat / iroN / iroE / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA	1..139741	139741

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-35)

Sequences

Toxin

Protein

• Length: 41 a.a.
• Molecular weight: 4536.35 Da
• Isoelectric Point: 8.2691

>T277433 WP_223195199.1 NZ_CP122633:105860-105982 [Escherichia coli]
IVCCTLLIFTLLTRNRLCEVRLKDGYREVTASLAYESSGK

Nucleotide

• Length: 123 bp

Antitoxin

Nucleotide

• Length: 212 bp

>AT277433 NZ_CP122633:105557-105768 [Escherichia coli]
TCACACGGATTACCCGTGAACTGGTTGAACGACCAGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
AGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGC
AGAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGCCCCCTGTATGTCT

Structures

Antitoxin