Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4219937..4220631 Replicon chromosome
Accession NZ_CP122620
Organism Escherichia coli strain ETEC4090

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag QDW69_RS21495 Protein ID WP_001263489.1
Coordinates 4219937..4220335 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag QDW69_RS21500 Protein ID WP_000554758.1
Coordinates 4220338..4220631 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (4215525) 4215525..4215605 - 81 NuclAT_13 - -
- (4215525) 4215525..4215605 - 81 NuclAT_13 - -
- (4215525) 4215525..4215605 - 81 NuclAT_13 - -
- (4215525) 4215525..4215605 - 81 NuclAT_13 - -
QDW69_RS21470 (4216201) 4216201..4216659 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
QDW69_RS21475 (4216920) 4216920..4218377 + 1458 WP_230905590.1 cytosol nonspecific dipeptidase -
QDW69_RS21480 (4218434) 4218434..4218955 - 522 Protein_4205 peptide chain release factor H -
QDW69_RS21485 (4218951) 4218951..4219157 - 207 Protein_4206 RtcB family protein -
QDW69_RS21490 (4219475) 4219475..4219927 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
QDW69_RS21495 (4219937) 4219937..4220335 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
QDW69_RS21500 (4220338) 4220338..4220631 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
QDW69_RS21505 (4220683) 4220683..4221738 - 1056 WP_001226164.1 DNA polymerase IV -
QDW69_RS21510 (4221809) 4221809..4222594 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
QDW69_RS21515 (4222566) 4222566..4224278 + 1713 Protein_4212 flagellar biosynthesis protein FlhA -
QDW69_RS21520 (4224502) 4224502..4224999 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T277368 WP_001263489.1 NZ_CP122620:c4220335-4219937 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT277368 WP_000554758.1 NZ_CP122620:c4220631-4220338 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References