Detailed information of TA system TA027732

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 35371..35937
• Replicon: chromosome
• Accession: NC_017846
• Organism: Aggregatibacter actinomycetemcomitans D7S-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: X2JU35
• Locus tag: D7S_RS00185
• Protein ID: WP_005543444.1
• Coordinates: 35659..35937 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: D7S_RS00180
• Protein ID: WP_005543446.1
• Coordinates: 35371..35643 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D7S_RS00150	31909..32175	-	267	WP_005543457.1	oxaloacetate decarboxylase subunit gamma	-
D7S_RS00155	32536..32727	+	192	WP_005553960.1	hypothetical protein	-
D7S_RS00160	32702..33079	+	378	WP_005543455.1	hypothetical protein	-
D7S_RS00165	33200..33475	-	276	WP_005543453.1	hydrogenase maturation factor HybG	-
D7S_RS11515	33505..33793	-	289	Protein_32	[NiFe]-hydrogenase assembly chaperone HybE	-
D7S_RS00175	33932..35362	-	1431	WP_005543449.1	bifunctional indole-3-glycerol-phosphate synthase TrpC/phosphoribosylanthranilate isomerase TrpF	-
D7S_RS00180	35371..35643	-	273	WP_005543446.1	HigA family addiction module antidote protein	Antitoxin
D7S_RS00185	35659..35937	-	279	WP_005543444.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D7S_RS00190	36032..37033	-	1002	WP_005543441.1	anthranilate phosphoribosyltransferase	-
D7S_RS00195	37045..37437	-	393	WP_005543439.1	tautomerase family protein	-
D7S_RS00200	37511..38101	-	591	WP_005543437.1	aminodeoxychorismate/anthranilate synthase component II	-
D7S_RS00205	38112..39659	-	1548	WP_005543435.1	anthranilate synthase component 1	-
D7S_RS00210	39825..40379	-	555	WP_005543432.1	HAD family hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-79)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10490.95 Da
• Isoelectric Point: 9.5983

>T27732 WP_005543444.1 NC_017846:c35937-35659 [Aggregatibacter actinomycetemcomitans D7S-1]
MILSFKHKGLEQFFKTGSTAGIQAKHAKKLNLQLATLNNAETPLAMSVPSWNLHKLKGDLQEHWAVTVNGNWRLTFKFEN
GHAEVVDYQDYH

Nucleotide

• Length: 279 bp

>T27732 NC_017846:c35937-35659 [Aggregatibacter actinomycetemcomitans D7S-1]
ATGATTTTATCTTTCAAACATAAGGGACTTGAACAATTTTTTAAAACCGGTTCGACCGCAGGAATTCAAGCCAAACACGC
CAAGAAATTAAATCTGCAATTGGCCACATTAAATAATGCGGAAACGCCTCTTGCTATGAGTGTTCCCAGCTGGAATTTGC
ACAAACTTAAAGGTGATTTGCAAGAGCATTGGGCAGTTACCGTAAACGGAAATTGGCGCTTAACATTTAAATTTGAAAAT
GGTCATGCTGAAGTTGTTGATTATCAGGATTATCATTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10138.74 Da
• Isoelectric Point: 7.3169

>AT27732 WP_005543446.1 NC_017846:c35643-35371 [Aggregatibacter actinomycetemcomitans D7S-1]
MHNPPHPGELLKEYIDGVSVTKVAQKLGVSRVTLSRILNGKASITPEMAVRLSELLNTTTPKLWLGMQADYDLWQIEQQH
AVFNIQPLFN

Nucleotide

• Length: 273 bp

>AT27732 NC_017846:c35643-35371 [Aggregatibacter actinomycetemcomitans D7S-1]
ATGCACAATCCTCCACACCCGGGAGAACTGTTAAAAGAATATATTGATGGCGTGAGTGTCACGAAGGTTGCCCAAAAATT
AGGTGTTTCGCGTGTTACGCTTTCCCGCATTCTTAATGGGAAAGCAAGTATAACGCCTGAAATGGCTGTGCGATTAAGCG
AATTATTGAATACCACAACACCGAAATTATGGCTGGGTATGCAAGCAGACTACGATTTATGGCAAATTGAACAACAACAC
GCCGTATTCAACATCCAACCATTATTTAATTAA

Structures

Toxin

• AlphaFold DB: X2JU35