Detailed information of TA system TA027727

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 453460..454178
• Replicon: chromosome
• Accession: NC_017845
• Organism: Pectobacterium sp. SCC3193

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: W5S_RS02100
• Protein ID: WP_043898902.1
• Coordinates: 453460..453765 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A0H3HZE9
• Locus tag: W5S_RS02105
• Protein ID: WP_014698585.1
• Coordinates: 453762..454178 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
W5S_RS02070	448569..449303	+	735	WP_014698579.1	type I-E CRISPR-associated protein Cas5/CasD	-
W5S_RS02075	449303..449950	+	648	WP_014698580.1	type I-E CRISPR-associated protein Cas6/Cse3/CasE	-
W5S_RS02080	449978..450895	+	918	WP_012822191.1	type I-E CRISPR-associated endonuclease Cas1	-
W5S_RS02085	450896..451189	+	294	WP_014698581.1	type I-E CRISPR-associated endoribonuclease Cas2	-
W5S_RS02090	452603..452974	-	372	WP_014698583.1	helix-turn-helix domain-containing protein	-
W5S_RS02095	453048..453308	-	261	WP_012822193.1	type II toxin-antitoxin system RelE/ParE family toxin	-
W5S_RS02100	453460..453765	+	306	WP_043898902.1	type II toxin-antitoxin system HigB family toxin	Toxin
W5S_RS02105	453762..454178	+	417	WP_014698585.1	helix-turn-helix domain-containing protein	Antitoxin
W5S_RS02110	455459..455917	+	459	WP_012822197.1	hypothetical protein	-
W5S_RS02115	455998..457350	-	1353	WP_014698586.1	aspartate aminotransferase family protein	-
W5S_RS02120	457381..458652	-	1272	WP_043898904.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (18-91)

Antitoxin

• Cro/C1-type helix-turn-helix domain (71-136)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 12068.82 Da
• Isoelectric Point: 9.8534

>T27727 WP_043898902.1 NC_017845:453460-453765 [Pectobacterium parmentieri]
VFSRAPFDTAIAQFPNQAAALADLYRTIKREIYATPDDMKKRFPSLDRMKYREKWWVIDIGGGHLRVMFFADFERGKIFI
KHITSHAEYDSLTEYYRRNKE

Nucleotide

• Length: 306 bp

>T27727 NC_017845:453460-453765 [Pectobacterium parmentieri]
GTGTTCTCCCGTGCACCTTTTGACACTGCAATCGCCCAATTCCCTAATCAGGCGGCAGCATTGGCTGACCTATACCGGAC
GATAAAGCGTGAAATATATGCAACGCCGGACGATATGAAAAAACGTTTCCCCAGCTTAGACAGGATGAAGTACAGGGAAA
AGTGGTGGGTTATTGATATTGGTGGCGGGCATCTTCGGGTGATGTTTTTCGCTGATTTTGAGCGAGGGAAAATTTTTATC
AAACACATCACATCCCATGCAGAGTACGACAGTCTGACAGAATATTACCGGAGGAATAAAGAATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15355.64 Da
• Isoelectric Point: 4.5667

>AT27727 WP_014698585.1 NC_017845:453762-454178 [Pectobacterium parmentieri]
MMYADAIKAANNLTSIVPLLGGSTSRKDYEDALKLTEYLLESEPDSPLIDMLTAKIDKYEEDAPEFVAFNERIKALPAGI
ALLRIPMDQHGLTQSDFEAEIGKKSLVSRILNGERTLTLDHMRALAKRFDIPVSAFID

Nucleotide

• Length: 417 bp

>AT27727 NC_017845:453762-454178 [Pectobacterium parmentieri]
ATGATGTACGCAGACGCAATCAAGGCCGCTAACAATCTGACGAGTATCGTACCGCTCCTCGGAGGTAGCACCTCTCGTAA
AGATTATGAGGATGCGCTGAAACTCACTGAATATTTACTCGAGAGCGAGCCTGATAGCCCGCTAATCGATATGCTGACGG
CTAAAATTGACAAATATGAAGAGGATGCGCCAGAGTTTGTAGCTTTCAACGAGCGTATTAAAGCGCTCCCTGCTGGTATC
GCTTTGCTGCGCATACCTATGGATCAGCATGGGCTAACGCAAAGTGACTTTGAAGCTGAGATCGGTAAAAAATCTCTGGT
TTCCCGCATTCTAAATGGTGAGCGTACACTAACGCTTGATCACATGAGAGCACTTGCTAAACGATTTGATATTCCCGTGA
GTGCTTTTATTGATTAG

Structures

Antitoxin

• AlphaFold DB: A0A0H3HZE9