Detailed information of TA system TA027718

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 57993..58501
• Replicon: plasmid PRA2
• Accession: NC_017773
• Organism: Rahnella aquatilis HX2

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: H8P157
• Locus tag: Q7S_RS26340
• Protein ID: WP_013578404.1
• Coordinates: 58235..58501 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: H8P156
• Locus tag: Q7S_RS25615
• Protein ID: WP_013578405.1
• Coordinates: 57993..58238 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Q7S_RS25595	53495..54955	-	1461	WP_014683042.1	sensor domain-containing diguanylate cyclase	-
Q7S_RS25600	55341..55679	-	339	Protein_51	MFS transporter	-
Q7S_RS25605	55804..56064	+	261	WP_014683044.1	DUF1778 domain-containing protein	-
Q7S_RS26335	56061..56540	+	480	WP_014683045.1	GNAT family N-acetyltransferase	-
Q7S_RS25610	56632..57681	-	1050	WP_014683046.1	tyrosine-type recombinase/integrase	-
Q7S_RS25615	57993..58238	+	246	WP_013578405.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
Q7S_RS26340	58235..58501	+	267	WP_013578404.1	Txe/YoeB family addiction module toxin	Toxin
Q7S_RS26350	59699..59863	-	165	WP_081481124.1	DUF1018 domain-containing protein	-
Q7S_RS26575	59879..60058	-	180	Protein_58	Cro/Cl family transcriptional regulator	-
Q7S_RS25630	60330..61274	-	945	WP_014683049.1	aminoimidazole riboside kinase	-
Q7S_RS25635	61391..62404	-	1014	WP_014683050.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..75160	75160

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (1-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10490.04 Da
• Isoelectric Point: 10.0392

>T27718 WP_013578404.1 NC_017773:58235-58501 [Rahnella aquatilis HX2]
MKTRFTKRGAADYQYWQGSNAKIFERIRILIHAIHADPLGGIGKPERLRHYKNPALYSRRIDREHRLVYSMVNDELTIIA
CRYHYDDV

Nucleotide

• Length: 267 bp

>T27718 NC_017773:58235-58501 [Rahnella aquatilis HX2]
ATGAAAACGCGATTCACTAAGAGAGGGGCAGCCGATTATCAATACTGGCAGGGAAGTAACGCCAAAATATTCGAGCGCAT
CCGTATACTGATACATGCAATTCATGCCGACCCACTGGGCGGTATTGGTAAGCCAGAACGGTTGCGCCATTATAAAAATC
CGGCACTTTATTCCCGGCGCATAGACAGAGAACACCGACTTGTTTACAGCATGGTAAATGATGAATTAACTATAATTGCC
TGCCGGTATCATTATGACGATGTTTAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9027.06 Da
• Isoelectric Point: 4.1947

>AT27718 WP_013578405.1 NC_017773:57993-58238 [Rahnella aquatilis HX2]
MHSLTFTDARKHFAETMQQVSDNAEPVRILRRDAPDVVLIDAAEYDAILETLYLFSNPANAAHINASLAELDRGDTVEIE
V

Nucleotide

• Length: 246 bp

>AT27718 NC_017773:57993-58238 [Rahnella aquatilis HX2]
ATGCATTCATTAACGTTTACTGATGCCCGTAAGCATTTTGCTGAGACAATGCAGCAGGTTAGCGACAATGCCGAGCCCGT
GCGTATTCTGCGACGTGATGCTCCTGATGTTGTACTGATTGACGCCGCCGAATATGACGCCATTTTAGAAACACTATATT
TATTCAGCAATCCTGCGAACGCTGCACATATTAACGCATCGCTGGCTGAGTTAGATCGCGGTGACACTGTCGAAATTGAA
GTATGA

Structures

Toxin

• AlphaFold DB: H8P157

Antitoxin

• AlphaFold DB: H8P156