Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3702726..3703267 Replicon chromosome
Accession NZ_CP122517
Organism Escherichia coli strain MY1-8

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag QCL60_RS17975 Protein ID WP_000615976.1
Coordinates 3702989..3703267 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag QCL60_RS17970 Protein ID WP_000729704.1
Coordinates 3702726..3702986 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QCL60_RS17945 (3698509) 3698509..3699294 - 786 WP_000207575.1 putative lateral flagellar export/assembly protein LafU -
QCL60_RS17950 (3699266) 3699266..3700978 + 1713 Protein_3512 flagellar biosynthesis protein FlhA -
QCL60_RS17955 (3701083) 3701083..3701361 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
QCL60_RS17960 (3701354) 3701354..3701710 + 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
QCL60_RS17965 (3701767) 3701767..3702540 - 774 WP_000543895.1 C40 family peptidase -
QCL60_RS17970 (3702726) 3702726..3702986 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
QCL60_RS17975 (3702989) 3702989..3703267 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
QCL60_RS17980 (3703423) 3703423..3704163 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
QCL60_RS17985 (3704134) 3704134..3704901 - 768 WP_000333380.1 class II glutamine amidotransferase -
QCL60_RS17990 (3705107) 3705107..3705685 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA / rhs/PAAR 3701354..3718171 16817


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T277151 WP_000615976.1 NZ_CP122517:3702989-3703267 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT277151 WP_000729704.1 NZ_CP122517:3702726-3702986 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References