Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4111519..4112246 Replicon chromosome
Accession NZ_CP122454
Organism Escherichia coli strain 82.0311

Toxin (Protein)


Gene name higB Uniprot ID Q3YYE6
Locus tag QBY76_RS20035 Protein ID WP_000547563.1
Coordinates 4111935..4112246 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag QBY76_RS20030 Protein ID WP_000126304.1
Coordinates 4111519..4111938 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QBY76_RS20015 (4107682) 4107682..4109934 - 2253 WP_085455499.1 carbamoyltransferase HypF -
QBY76_RS20020 (4110087) 4110087..4110614 - 528 WP_001078777.1 electron transport protein HydN -
QBY76_RS20025 (4110999) 4110999..4111427 + 429 WP_000536064.1 hypothetical protein -
QBY76_RS20030 (4111519) 4111519..4111938 - 420 WP_000126304.1 helix-turn-helix domain-containing protein Antitoxin
QBY76_RS20035 (4111935) 4111935..4112246 - 312 WP_000547563.1 type II toxin-antitoxin system HigB family toxin Toxin
QBY76_RS20040 (4112409) 4112409..4113164 - 756 WP_001314074.1 hypothetical protein -
QBY76_RS20045 (4113207) 4113207..4113674 - 468 WP_021522200.1 hydrogenase maturation peptidase HycI -
QBY76_RS20050 (4113667) 4113667..4114077 - 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
QBY76_RS20055 (4114074) 4114074..4114841 - 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
QBY76_RS20060 (4114841) 4114841..4115383 - 543 WP_000493799.1 formate hydrogenlyase subunit HycF -
QBY76_RS20065 (4115393) 4115393..4117102 - 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12398.11 Da        Isoelectric Point: 9.7105

>T276934 WP_000547563.1 NZ_CP122454:c4112246-4111935 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15474.50 Da        Isoelectric Point: 4.4596

>AT276934 WP_000126304.1 NZ_CP122454:c4111938-4111519 [Escherichia coli]
MTANAVRAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLTSRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QA22

Antitoxin

Source ID Structure

References